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Determination of Midodrine in Human Plasma by High-Performance Liquid Chromatography with Fluorescence Detection

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Abstract

A simple and sensitive fluorometric high-performance liquid chromatographic method was developed for the determination of midodrine in human plasma. After liquid-liquid extraction from plasma, the drug and 2-phenylglycinol (internal standard) were converted into the corresponding fluorescent derivatives by reaction with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives were separated within 30 min on a reversed-phase column using isocratic elution with acetonitrile-methanol-water (10:30:60, v/v) and were detected spectrofluorometrically at 485 nm with excitation at 400 nm. The detection limit for midodrine was 0.3 pmol (76 pg) per mL plasma at a signal-to-noise ratio of 3.

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References

  1. O. Thlesius, Int. Eur. J. Clin. Pharmacol., 1979, 16, 423.

    Article  Google Scholar 

  2. H. Pittner, Gen. Pharmac., 1983, 14, 107.

    Article  CAS  Google Scholar 

  3. D. M. Tavish, Drugs, 1989, 38, 757.

    Article  Google Scholar 

  4. N. Kolossa, W. G. Schutzenberger, H. Wiener, and P. Krivanek, Arch. Int. Pharmacodyn., 1979, 238, 96.

    Google Scholar 

  5. S. Tsutsumi, T. Suwa, N. Masuda, T. Minagawa, M. Sano, T. Tamatani, and K. Tsuchida, Clin. Report, 1987, 21, 1895.

    Google Scholar 

  6. W. Posch and W. Lindner, Biomed. Chromatogr., 1989, 3, 153.

    Article  CAS  Google Scholar 

  7. M. G. Quagla, A. Farina, E. Bossú, and V. Cotichini, J. Pharm. Biomed. Anal., 1998, 18, 171.

    Article  Google Scholar 

  8. J. Ishida, M. Yamaguchi, T. Iwata, and M. Nakamura, Anal. Chim. Acta, 1989, 223, 319.

    Article  CAS  Google Scholar 

  9. J. Ishida, M. Yamaguchi, and M. Nakamura, Anal. Biochem., 1990, 184, 86.

    Article  CAS  Google Scholar 

  10. J. Ishida, M. Yamaguchi, and M. Nakamura, Anal. Biochem., 1991, 195, 168.

    Article  CAS  Google Scholar 

  11. T. Iwata, H. Fujino, J. Sonoda, and M. Yamaguchi, Anal. Sci., 1997, 13 (supplement), 467.

    Article  CAS  Google Scholar 

  12. T. Iwata, M. Yamaguchi, S. Hara, M. Nakamura, and Y. Ohkura, J. Chromatogr., 1986, 362, 209.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan, Fukuoka, 814-0180, Japan

    Hideyuki Yoshida, Yuki Ohno, Kenichiro Yoshikuni, Kenichiro Todoroki, Hitoshi Nohta & Masatoshi Yamaguchi

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  1. Hideyuki Yoshida
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  2. Yuki Ohno
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  3. Kenichiro Yoshikuni
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  4. Kenichiro Todoroki
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  5. Hitoshi Nohta
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  6. Masatoshi Yamaguchi
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Corresponding author

Correspondence to Masatoshi Yamaguchi.

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Yoshida, H., Ohno, Y., Yoshikuni, K. et al. Determination of Midodrine in Human Plasma by High-Performance Liquid Chromatography with Fluorescence Detection. ANAL. SCI. 19, 317–319 (2003). https://doi.org/10.2116/analsci.19.317

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  • DOI: https://doi.org/10.2116/analsci.19.317

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