Bead-injection Determination of Total Mercury in River Water Samples

Abstract

A bead-injection system is proposed for total mercury determination in river-water samples. The procedure is based on the introduction of a defined quantity of a resin suspension in the flow system. The selected beads are packed inside of a flow cell and the formed resin mini-column constitutes the optical path. The sample volume is then selected, and its passage by the mini-column allows retention of the mercury ions on the surfaces of the beads. The introduction of a spectrophotometric reagent in the flow system leads to the formation of a colored Hg-dithizone complex on the surface of the bead, which is spectrophotometricaly monitored. The spent beads are directed to waste, allowing the system to become ready to process another sample. The proposed system handles about 20 measurements per hour, consuming 1000 µl of the sample, 1 mg of Chelex 100 resin and 1.25 µg of Dithizone per determination. When 1000 µl of the sample is injected, a linear analytical curve is obtained (A = 0.0052[Hg] + 0.1028, from 0 up to 30 µg l^-1, R² = 0.995); the detection limit is estimated to be 0.9 µg l^-1. The results are precise, r.s.d. < 9%; spiked sample recoveries within 91.2 and 109% are found.