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Development and Evaluation of a GC/FID Method for the Analysis of Free Amino Acids in Quince Fruit and Jam

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Abstract

A GC/FID methodology for determination of twenty-one free amino acids in quince fruit (pulp and peel) and jam is described. The sample preparation was simple, ntinvolving a SCX Solid-Phase Extraction (SPE) purification step and a fast derivatization with ethyl chloroformate for gas chromatographic analysis. The chromatographic separation was achieved using a CP-Sil 19 CB wcot fused-silica capillary column. Under the chosen conditions, with temperature and pressure programming, this capillary column was able to separate all the amino acids not only in a short time but also with good separation. The GC/FID procedure is rapid, sensitive, reproducible and accurate. The detection limit values for amino acids were low, between 0.004 and 0.115 µg/mL, and the method was precise. As a general rule, the recovery values were high. Due to its rapidity and low cost, this technique can be useful in the quality control of quince products.

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Authors and Affiliations

  1. REQUIMTE, Serviço de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 4050-047, Porto, Portugal

    Branca M. Silva, Paula B. Andrade & Rosa M. Seabra

  2. REQUIMTE, Serviço de Bromatologia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 4050-047, Porto, Portugal

    Susana Casal, M. Beatriz Oliveira & Margarida A. Ferreira

Authors
  1. Branca M. Silva
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  2. Susana Casal
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  3. Paula B. Andrade
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  4. Rosa M. Seabra
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  5. M. Beatriz Oliveira
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  6. Margarida A. Ferreira
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Corresponding author

Correspondence to Rosa M. Seabra.

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† To whom correspondence should be addressed.

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Silva, B.M., Casal, S., Andrade, P.B. et al. Development and Evaluation of a GC/FID Method for the Analysis of Free Amino Acids in Quince Fruit and Jam. ANAL. SCI. 19, 1285–1290 (2003). https://doi.org/10.2116/analsci.19.1285

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  • DOI: https://doi.org/10.2116/analsci.19.1285

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