Abstract
A pressure-controlled on-column injection method was developed for microcolumn liquid chromatography. The system was assembled from a syringe pump, a Model M-445 Six-Way Micro Selection Valve, a separation column and a UV detector. The injection volume could be regulated by changing the applied pressure and/or the sample loading time. The system was evaluated in the ion-exchange mode. The system was applied to the determination of anions in river-water samples.
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References
D. Ishii, Jasco Report, 1974, 11(6), 1.
R. P. W. Scott and P. Kucera, J. Chromatogr., 1976, 125, 251.
K. Hibi, D. Ishii, I. Fujishima, T. Takeuchi, and T. Nakanishi, J. High Resolut. Chromatogr. Chromatogr. Commun., 1978, 1, 21.
T. Tsuda and M. V. Novotny, Anal. Chem., 1978, 50, 632.
J. W. Jorgenson and E. J. Guthrie, J. Chromatogr., 1983, 255, 335.
K. Jinno, Chromatographia, 1988, 25, 1004.
J. E. MacNair, K. C. Lewis, and J. W. Jorgenson, Anal. Chem., 1997, 69, 983.
T. F. Hooker and J. W. Jorgenson, Anal. Chem., 1997, 69, 4134.
F. E. Regnier, J. High Resolut. Chromatogr., 2000, 23, 19.
N. Tanaka, H. Nagayama, H. Kobayashi, T. Ikegami, K. Hosoya, N. Ishizuka, H. Minakuchi, K. Nakanishi, K. Cabrera, and D. Lubda, J. High Resolut. Chromatogr., 2000, 23, 111.
T. Takeuchi, J. Anal. Bioanal. Chem., 2003, 375, 25.
T. Takeuchi and D. Ishii, J. Chromatogr., 1981, 218, 199.
H. Terada, M. Asanoma, and Y. Sakabe, J. Chromatogr., 1985, 318, 299.
T. Takeuchi, Y. Jin, and D. Ishii, J. Chromatogr., 1985, 321, 159.
H. W. Mueller and J. Eitel, J. Chromatogr. B, 1996, 678, 137.
M. A. Rezai, G. Famiglini, and A. Cappiello, J. Chromatogr. A, 1996, 742, 69.
A. Cappiello, G. Famiglini, and A. Berloni, J. Chromatogr. A, 1997, 768, 215.
M. D. Foster, M. A. Arnold, J. A. Nichols, and S. R. Bakalyar, J. Chromatogr. A, 2000, 869, 231.
L. Lim, T. Miwa, and T. Takeuchi, Anal. Sci., 2001, 17, i887.
T. Takeuchi and D. Ishii, J. Chromatogr., 1981, 213, 25.
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Takeuchi, T., Kimura, T., Jin, JY. et al. Pressure-Controlled On-column Injection Method for Microcolumn Liquid Chromatography. ANAL. SCI. 19, 1265–1267 (2003). https://doi.org/10.2116/analsci.19.1265
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DOI: https://doi.org/10.2116/analsci.19.1265