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Influence of Culture Conditions on Cell Proliferation in a Microfluidic Channel

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Abstract

Microfluidic devices have emerged as a new cell culture tool, which can mimic the structure and physiology of living human organs. However, no standardized culture method for a microfluidic device has yet been established. Here, we describe the effects of various conditions on cell proliferation in a microchannel with a depth smaller than 100 μm. Primary endothelial cell proliferation was suppressed with a decrease in the culture medium volume per cell culture area. Moreover, cell growth was compared with or without medium flow, and the optimum culture condition was determined to be 1 μL/h flow in a 65-μm-deep microchannel. In addition, glucose consumption was greater under fluidic conditions than under static conditions, and the ability of tumor (HeLa) cells to convert glucose into lactate appeared to be higher in a static culture than that in a fluidic culture. Overall, our results will serve as a useful guide for designing a microfluidic cell culture platform in a channel smaller than 100 μm.

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Acknowledgments

This study was supported in part by a Banyu Foundation Research Grant, the Science Research Promotion Fund, Suzuken Memorial Foundation, Shiseido Female Researcher Science Grant, Koyanagi Foundation Research Grant, and a Grant-in- Aid for Scientific Research (Japan Society for the Promotion of Science, KAKENHI; Grant Nos. 25600065 and 16H04170).

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Authors and Affiliations

  1. Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, 112-8681, Japan

    Kae Sato, Miwa Sato, Mizuho Yokoyama, Mai Hirai & Aya Furuta

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  1. Kae Sato
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  2. Miwa Sato
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  3. Mizuho Yokoyama
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  4. Mai Hirai
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  5. Aya Furuta
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Correspondence to Kae Sato.

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Sato, K., Sato, M., Yokoyama, M. et al. Influence of Culture Conditions on Cell Proliferation in a Microfluidic Channel. ANAL. SCI. 35, 49–56 (2019). https://doi.org/10.2116/analsci.18SDP04

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  • DOI: https://doi.org/10.2116/analsci.18SDP04

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