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Changes in Polyamine Content in Rice Bran due to Fermentation with Aspergillus oryzae Analyzed by LC/ESI-MS/MS Combined with Derivatization

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Abstract

Many studies have demonstrated that the dietary supplementation of polyamines, especially spermidine (SPD), prevents age-related diseases. Rice bran is rich in polyamines and their amounts could be increased by fermentation with Aspergillus oryzae (A. oryzae). In this study, we developed a method for the determination of putrescine (PUT), SPD and spermine (SPM) in rice bran samples by liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI-MS/MS) after derivatization with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F). The derivatization improved the LC retention and ESI-MS/MS detectability of the polyamines, and consequently enabled precise and accurate quantification. Using this method, we found that the SPD content increased to 158% due to fermentation with A. oryzae, while the content of PUT and SPM decreased. SPD is known as the polyamine playing a central role in cell proliferation and growth, and therefore has health benefits. The fermented rice bran might be a good material for functional foods aimed at SPD supplementation.

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Acknowledgments

This study was supported in part by research funding from Genmai Koso Co., Ltd. (Sapporo, Japan). The funding sponsor had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Yukiko Horie, Ayaka Goto, Sumi Tsubuku, Shoujiro Ogawa & Tatsuya Higashi

  2. Research and Development Division, Koken Co., Ltd., 329 Nakagoya, Tobetsu, Hokkaido, 061-0211, Japan

    Yukiko Horie & Mari Itoh

  3. Genmai Koso Co., Ltd., 1-1, Kita 12, Nishi 1, Kita, Sapporo, Hokkaido, 001-0012, Japan

    Shigeo Ikegawa

Authors
  1. Yukiko Horie
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  2. Ayaka Goto
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  3. Sumi Tsubuku
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  4. Mari Itoh
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  5. Shigeo Ikegawa
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  6. Shoujiro Ogawa
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  7. Tatsuya Higashi
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Correspondence to Tatsuya Higashi.

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Horie, Y., Goto, A., Tsubuku, S. et al. Changes in Polyamine Content in Rice Bran due to Fermentation with Aspergillus oryzae Analyzed by LC/ESI-MS/MS Combined with Derivatization. ANAL. SCI. 35, 427–432 (2019). https://doi.org/10.2116/analsci.18P483

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  • DOI: https://doi.org/10.2116/analsci.18P483

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