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Development of a Microfluidic System Comprising Dialysis and Secretion Components for a Bioassay of Renal Clearance

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Abstract

We have developed a microfluidic bioassay system that mimics glomerular filtration and tubular secretion in the kidney. The system consists of a peristaltic micropump (heart), a dialysis component (renal corpuscle), and a secretion component (renal proximal tubule). Analytes were separated by size using a dialysis membrane in the dialysis component. Model cells were cultured on a membrane in the secretion component, and active transport mediated by P-glycoprotein (P-gp) was confirmed using the P-gp substrate rhodamine 123 with or without the P-gp inhibitor quinidine sulfate. The system achieved both size separation and selective transport by P-gp on a single microchip. This proof-of-concept model may find applications in drug excretion assays, including studies of drug-drug interactions during tubular secretion.

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Acknowledgments

This work was supported in part by the Asahi Glass Foundation and the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant Number JP15H03823).

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Authors and Affiliations

  1. School of Science and Technology, Gunma University, 1-5-1 Tenjincho, Kiryu, Gunma, 376-8515, Japan

    Yu Sakuta, Kin-Ichi Tsunoda & Kiichi Sato

  2. Daiichi Sankyo Co., Ltd., 1-2-58 Hiromachi, Shinagawa, Tokyo, 140-8710, Japan

    Issey Takehara

Authors
  1. Yu Sakuta
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  2. Issey Takehara
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  3. Kin-Ichi Tsunoda
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  4. Kiichi Sato
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Correspondence to Kiichi Sato.

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Sakuta, Y., Takehara, I., Tsunoda, KI. et al. Development of a Microfluidic System Comprising Dialysis and Secretion Components for a Bioassay of Renal Clearance. ANAL. SCI. 34, 1073–1078 (2018). https://doi.org/10.2116/analsci.18P141

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  • DOI: https://doi.org/10.2116/analsci.18P141

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