Skip to main content
SpringerLink
Log in

Melanin Radicals in Paraffin-embedded Melanoma Investigated Using Surface-type Dielectric Resonator for X-band EPR

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

We investigated melanin radicals in paraffin-embedded malignant melanoma (MM) using a surface-type dielectric resonator for X-band electron paramagnetic resonance (EPR) and analyzed the radical species. The surface-type resonator’s performance was examined using 5–10 μL of 0.1 mM 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL) aqueous solution in a 1.0-mm (i.d.) glass capillary as well as 1,1-diphenyl-2-picrylhydrazyl (DPPH) powder. The surface-type detection has approximately two times poorer S/N ratio than commercial insertion-type detection. A sample of the paraffin-embedded MM specimen was used for the radical detection. We obtained an EPR spectrum of melanin radicals in the paraffin-embedded melanoma sample (size ∼3 × 4 × 3 mm). A single line (∼0.64 mT peak-topeak line-width) with a small shoulder was observed and was identified as a pheomelanin-related radical. The pheomelanin radical can be directly related to the MM. Thus, the present results were a good indication for noninvasive measurement, as well as for detailed analyses of melanin radicals in human MM.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. He, A. Samouilov, P. Kuppusamy, and J. L. Zweier, J. Magn. Reson., 2001, 148, 155.

    Article  CAS  PubMed  Google Scholar 

  2. H. Hirata, T. Walczak, and H. M. Swartz, J. Magn. Reson., 2000, 142, 159.

    Article  CAS  PubMed  Google Scholar 

  3. K. Nakagawa, Y. Ohba, B. Epel, and H. Hirata, J. Oleo. Sci., 2012, 61, 451.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. K. Nakagawa, Spectrochim. Acta, Part A, 2015, 150, 461.

    Article  CAS  Google Scholar 

  5. K. Nakagawa, Japanese Patent, submitted, 2016, #2016–158210.

    Google Scholar 

  6. H. Sugawara, H. Hirata, S. Petryakov, P. Lesniewski, B. B. Williams, A. B. Flood, and H. M. Swartz, IEEE Trans. Biomed. Eng., 2014, 61, 1894.

    Article  PubMed  Google Scholar 

  7. S. V. Petryakov, W. Schreiber, M. M. Kmiec, B. B. Williams, and H. M. Swartz, Radiat. Prot. Dosimetry, 2016, 172, 127.

    Article  PubMed  PubMed Central  Google Scholar 

  8. a) K. Nakagawa, S. Minakawa, and D. Sawamura, Hirosaki University Internal Review Board approval. 2011, 004. b) S. Minakawa, Y. Matsuzaki, T. Kaneko, K. Nakagawa, and D. Sawamura, Hirosaki University Internal Review Board approval. 2014, 390.

    Google Scholar 

  9. a) K. Nakagawa, S. Minakawa, D. Sawamura, and H. Hara, Anal. Sci., 2017, 33, 1357. b) Q. Godechal, P. Leveque, L. Marot, J-F. Baurain, and B. Gallez, Exp. Dermatol., 2012, 21, 341.

    Article  CAS  PubMed  Google Scholar 

  10. S. Petryakov, M. Chzhan, A. Samouilov, G. He, P. Kuppusamy, and J. L. Zweier, J. Magn. Reson., 2001, 151, 124.

    Article  CAS  PubMed  Google Scholar 

  11. A. Slominski, P. M. Plonka, A. Pisarchik, J. L. Smart, V. Tolle, J. Wortsman, and M. J. Low, Endocrinology, 2005, 146, 1245.

    Article  CAS  PubMed  Google Scholar 

  12. E. Chikvaidze and I. Khachatryan, Int. J. Cosme. Sci., 2011, 33, 322.

    Article  CAS  Google Scholar 

  13. I. Salikhov, H. Hirata, T. Walczak, and H. M. Swartz, J. Magn. Reson., 2003, 164, 54.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

Part of this research was supported by KAKENHI Grant number (JP15K12499) from the Japan Society for the Promotion of Science (JSPS) (K. N.) and the Cooperative Research Program of Network Joint Research Center for Materials and Devices (K. N.).

Author information

Authors and Affiliations

  1. Division of Regional Innovation, Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki, 036?8564, Japan

    Kouichi Nakagawa

  2. Department of Dermatology, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, 036?8562, Japan

    Satoko Minakawa & Daisuke Sawamura

Authors
  1. Kouichi Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Satoko Minakawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daisuke Sawamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kouichi Nakagawa.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nakagawa, K., Minakawa, S. & Sawamura, D. Melanin Radicals in Paraffin-embedded Melanoma Investigated Using Surface-type Dielectric Resonator for X-band EPR. ANAL. SCI. 34, 837–840 (2018). https://doi.org/10.2116/analsci.18P055

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.18P055

Keywords

Search

Navigation