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Umbelliferone as a Small Molecular Peroxidase Mimic towards Sensitive Detection of H2O2 and Glucose

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Abstract

In this work, umbelliferone, a kind of coumarin derivative, was proved to exhibit peroxidase-like activity that could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide to generate a blue-colored oxide (oxTMB). The catalytic mechanism is similar to that of native enzymes (e.g. horseradish peroxidase, HRP) and nanozymes, which follow the Michaelis–Menten kinetics behavior. Meanwhile, the 7-hydroxyl group of umbelliferone plays a significant role in the peroxidase-like activity. Compared with enzymes and nanozymes, this small molecular mimic enzyme possesses the advantages of low cost, simple molecular structures, small molecular weight and high stability against harsh conditions. Based on the favorable peroxidase mimetic activity of umbelliferone, a convenient, practical and sensitive H2O2 and glucose detection method was successfully established. This work not only opens some new inspirations into seeking for novel molecular enzyme mimetics with excellent catalytic activities, but also provides promising assays for clinical diagnosis.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. 21405124, 21175110), the Fundamental Research Funds for the Central Universities (No. XDJK2013A022).

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Authors and Affiliations

  1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry’ (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, 400715, China

    Chaoqun Sun, Zili Huang, Li Liu, Menglu Li & Huzhi Zheng

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  1. Chaoqun Sun
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  2. Zili Huang
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  3. Li Liu
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  4. Menglu Li
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  5. Huzhi Zheng
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Correspondence to Huzhi Zheng.

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Sun, C., Huang, Z., Liu, L. et al. Umbelliferone as a Small Molecular Peroxidase Mimic towards Sensitive Detection of H2O2 and Glucose. ANAL. SCI. 34, 933–938 (2018). https://doi.org/10.2116/analsci.18P023

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  • DOI: https://doi.org/10.2116/analsci.18P023

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