Skip to main content
SpringerLink
Log in

A Novel Method for the Assay of α-Glucosidase Inhibitory Activity Using a Multi-channel Oxygen Sensor

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

The quantification of glucose by using a multi-channel dissolved oxygen (DO) meter (DOX96) with immobilized glucose oxidase (GOD) and mutarotase (MUT) was performed. An evaluation of the inhibitory activities for α-glucosidase (AGH) by modifying our batch-type pseudo-in vivo assay system [Oki et al.; Biol. Pharm. Bull., 2000, 232, 1084] was also performed using a DOX96. When 45 U/well GOD and 18.75 U/well MUT were immobilized on the surface of a gelatin membrane on the electrodes, the response shown by the decrease percent of DO (%) obtained with 8 electrode wells in the same row was linear with the glucose concentration up to 3.3 mM and a correlation coefficient larger than 0.9. To estimate the AGH inhibitory activity, AGH-immobilized Sepharose supports in the well of a silent screen plate were used. The IC50 values of acarbose and 1-deoxynojirimycin, a medicinal inhibitor for diabetes, were 0.70 ± 0.08 µM and 0.40 ± 0.13 µM, respectively, and coincided well with those by a pseudo-in vivo assay.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Peters and F. M. Richards, Ann. Rev. Biochem., 1977, 46, 523.

    Article  CAS  Google Scholar 

  2. S. Stanik and R. Marcus, Metabolism, 1980, 29, 346.

    Article  CAS  Google Scholar 

  3. H. Bischoff, Eur. J. Clin. Invest., 1994, 24(Supplement), 3.

    Article  CAS  Google Scholar 

  4. M. Toeller, Eur. J. Clin. Invest., 1994, 24(Supplement), 31.

    Article  Google Scholar 

  5. S. Chiba, Biosci. Biotech. Biochem., 1997, 61, 1233.

    Article  CAS  Google Scholar 

  6. T. Toyota, Horumon to Rinshou, 1995, 43, 51.

    Google Scholar 

  7. M. Miura and T. Gomyo, J. Jpn. Soc. Food Sci. Technol., 1996, 43, 157.

    Article  Google Scholar 

  8. T. Matsui, S. Yoshimoto, K. Osajima, T. Oki, and Y. Osajima, Biosci. Biotech. Biochem., 1996, 60, 2019.

    Article  CAS  Google Scholar 

  9. H. Odaka, N. Miki, H. Ikeda, and T. Matsuo, J. Jpn. Soc. Nutr. Food Sci., 1992, 45, 27.

    Article  CAS  Google Scholar 

  10. T. Oki, T. Matsui, and K. Matsumoto, Biol. Pharm. Bull., 2000, 232, 1084.

    Article  Google Scholar 

  11. G. Wagner and G. G. Guilbault (ed.), “Food Biosensor Analysis”, 1994, Marcel Dekker Inc., N. Y.

    Google Scholar 

  12. K. Matsumoto, H. Kamikado, H. Matsubara, and Y. Osajima, Anal. Chem., 1988, 60, 147.

    Article  CAS  Google Scholar 

  13. K. Matsumoto, H. Matsubara, M. Hamada, H. Ukeda, and Y. Osajima, J. Biotechnology, 1990, 14, 115.

    Article  CAS  Google Scholar 

  14. R. L. C. Chen and K. Matsumoto, Biosci. Biotech. Biochem., 1995, 59, 813.

    Article  CAS  Google Scholar 

  15. K. Matsumoto, T. Tsukatani, and S. Higuchi, Sens. Mater., 1995, 7, 167.

    CAS  Google Scholar 

  16. M.-H. Lee, R. L. C. Chen, and K. Matsumoto, Biosci. Biotech. Biochem., 1996, 60, 99.

    Article  CAS  Google Scholar 

  17. H. Ukeda, M. Ohira, and M. Sawamura, Anal. Sci., 1999, 15, 447.

    Article  CAS  Google Scholar 

  18. H. Ukeda, M. Ohira, K. Sarker, and M. Sawamura, Food Res. Int., 1998, 31, 297.

    Article  CAS  Google Scholar 

  19. H. Ukeda, Y. Fujita, M. Ohira, and M. Sawamura, J. Agric. Food Chem., 1996, 44, 3858.

    Article  CAS  Google Scholar 

  20. J. Arai, K. Yamada, M. Yasuda, M. Yoshida, T. Unten, M. Akamatsu, and H. Ishikawa, Human Cell, 1998, 11, 175.

    CAS  PubMed  Google Scholar 

  21. J. Arai and H. Kato, Electrochemistry, 1999, 67, 479.

    Article  CAS  Google Scholar 

  22. K. Matsumoto, K. Yamada, and Y. Osajima, Anal. Chem., 1981, 53, 1974.

    Article  CAS  Google Scholar 

  23. A. Cogoli, H. Mosimann, C. Vock, A.-K. Balthazar, and G. A. Semenza, Eur. J. Biochem., 1974, 30, 7.

    Article  Google Scholar 

  24. H.-O. Beutler, in “Methods of Enzymatic Analysis”, ed. H. U. Bergmeyer, 3rd ed., 1983, Vol. 6, Verlag Chemie, Weiheim, Deerfield Beach/Florida, Basel, 376.

Download references

Author information

Authors and Affiliations

  1. Laboratory of Food Analysis, Division of Food Biotechnology Department of Bioscience and Biotechnology Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan

    Kiyoshi Matsumoto, Kayoko Takemata, Kiyofumi Takayama, Kanthi J. M. Abesundara & Toshiro Matsui

  2. Daikin Industrial Co., Ltd., Miyukigaoka, Tsukuba, 305-0841, Japan

    Hideo Katayama

Authors
  1. Kiyoshi Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kayoko Takemata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kiyofumi Takayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kanthi J. M. Abesundara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toshiro Matsui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hideo Katayama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kiyoshi Matsumoto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsumoto, K., Takemata, K., Takayama, K. et al. A Novel Method for the Assay of α-Glucosidase Inhibitory Activity Using a Multi-channel Oxygen Sensor. ANAL. SCI. 18, 1315–1319 (2002). https://doi.org/10.2116/analsci.18.1315

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.18.1315

Search

Navigation