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Development of Titania Nanotube-based Electrochemical Immunosensor and Determination of Prostate Specific Antigen

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Abstract

Early diagnosis of cancer is the most important factor that increases the success of treatment. Therefore, the development of new diagnostic tools is a necessity. In this study, a new electrode surface was developed via modification of a disposable titanium electrode with anodic oxidation and coating of gold nanoparticle and chitosan. Titanium electrodes were anodized by several anodization parameters to obtain a nanoporous surface and characterized by scanning electron microscopy. Electrodes anodized in optimum conditions were modified with gold nanoparticles and chitosan for enhancing conductivity and functionalizing the surface of electrode, respectively. To detect prostate specific antigen (PSA), antiPSA was bound onto the functional electrode surface. Modified electrodes were characterized with scanning electron microscopy and cyclic voltammetry and used for chronoamperometric detection of PSA. Limit of detection (LOD) of the designed electrode was found to be 7.8 ng mL−1 for PSA in a linear range of 0–100 ng mL−1.

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Authors and Affiliations

  1. Bioengineering Division, Hacettepe University, Ankara, Turkey

    Damla Kiziltan & Emir Baki Denkbas

  2. Chemistry Department, Biochemistry Division, Hacettepe University, Ankara, Turkey

    Tayfun Vural, Serhat Ozturk & Emir Baki Denkbas

  3. Advanced Technologies Application and Research Center, HUNITEK, Hacettepe University, Ankara, Turkey

    Cem Bayram

  4. Nanotechnology and Nanomedicine Division, Hacettepe University, Ankara, Turkey

    Betul Bozdogan

  5. Chemistry Department, Analytical Chemistry Division, Hacettepe University, Ankara, Turkey

    Yesim Tugce Yaman & Serdar Abaci

Authors
  1. Damla Kiziltan
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  2. Tayfun Vural
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  3. Cem Bayram
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  4. Serhat Ozturk
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  5. Betul Bozdogan
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  6. Yesim Tugce Yaman
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  7. Serdar Abaci
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  8. Emir Baki Denkbas
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Correspondence to Emir Baki Denkbas.

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Kiziltan, D., Vural, T., Bayram, C. et al. Development of Titania Nanotube-based Electrochemical Immunosensor and Determination of Prostate Specific Antigen. ANAL. SCI. 34, 789–794 (2018). https://doi.org/10.2116/analsci.17P407

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  • DOI: https://doi.org/10.2116/analsci.17P407

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