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Application of an HPLC-FTIR Modified Thermospray Interface for Analysis of Dye Samples

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Abstract

Previously, we developed a reversed-phase HPLC method compatible to high performance liquid chromatography diffuse reflectance Fourier-transform infrared (HPLC-FTIR) thermospray interface for the analysis of dyes. Dye separation achieved with a mixed-mode (SCX-ODS) column using a small gradient (90 to 80% water with acetic acid) and pH 3.25; 10 to 20% acetonitrile was considered to be suitable for HPLC-FTIR. A constant-voltage setting for the thermospray temperature (227°C) was successfully used for this gradient condition. The HPLC-separated components deposited as a series of concentrated spots on a moving tape were scanned by specially developed HPLC-FTIR software. Excellent repeatability of the thermospray deposition FTIR chromatograms and IR spectra was obtained. The interface-derived spectra of the separated components of formulated and purified reactive dyes were compared and differences in spectral features were observed.

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Authors and Affiliations

  1. Department of Pure and Applied Chemistry, University of Strathclyde, Thomas Graham Building, 295 Cathedral Street, Glasgow, G1 1XL, United Kingdom

    Mohammad A. Mottaleb & David Littlejohn

  2. Department of Pharmacy, Dongduk Women’s University, 23-1 Sungbuk-ku, Seoul, 136-714, Korea

    Mohammad A. Mottaleb

Authors
  1. Mohammad A. Mottaleb
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  2. David Littlejohn
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Mottaleb, M.A., Littlejohn, D. Application of an HPLC-FTIR Modified Thermospray Interface for Analysis of Dye Samples. ANAL. SCI. 17, 429–434 (2001). https://doi.org/10.2116/analsci.17.429

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  • DOI: https://doi.org/10.2116/analsci.17.429

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