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Separation of Red Blood Cells at the Single Cell Level by Capillary Zone Electrophoresis

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Abstract

By using capillary zone electrophoresis, the human red blood cell population was separated at the single cell level. A single injection of a human blood cell sample gave about one hundred peaks, and most of the peaks could be used to estimate the approximate number of cells. The separation of human red blood cells was also detected by a direct microscopic observation. The injection of a very diluted blood solution gave a few tens of peaks; and the peak heights were almost equal. The peak profile in the electropherogram corresponds directly to the distribution of the electrophoretic mobility of the red blood cells, which reflects on the cell properties, such as the surface charge density, volume and weight. Our finding is the first report to present the fine separation of population of red blood cells at the single cell level.

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References

  1. S. Kitagawa, C. Kawaura, O. Hashimoto, M. Naoi, and T. Tsuda, Electrophoresis, 1995, 16, 1364.

    Article  CAS  Google Scholar 

  2. F. Foret, L. Krivanova, and P. Bocek, “Capillary Zone Electrophoresis”, 1993, VCH, Weinheim.

    Google Scholar 

  3. T. Matsue, N. Matsumoto, S. Koike, and I. Uchida, Biochi. Biophys, Acta, 1993, 1157, 332.

    Article  CAS  Google Scholar 

  4. Y. Miwa, S. Yoshimura, and A. Itoh, Int. J. Japan Soc. Prec. Eng., 1992, 26, 249.

    Google Scholar 

  5. S. Hjerten, K. Elenbring, F. Kilar, J. Liao, A. J. C. Chen, C. J. Siebert, and M. Zhu, J. Chromatogr., 1987, 403, 47.

    Article  CAS  Google Scholar 

  6. P. D. Grossman and D. S. Soane, Anal. Chem., 1990, 62, 1592.

    Article  CAS  Google Scholar 

  7. A. Zhu and Y. Chen, J. Chromatogr., 1989, 470, 251.

    Article  CAS  Google Scholar 

  8. S. Kitagawa, T. Tsuda, and O. Nozaki, Bunseki Kagaku, 1998, 47, 355.

    Article  CAS  Google Scholar 

  9. S. Kitagawa, O. Nozaki, and T. Tsuda, Electrophoresis, 1999, 20, 2560.

    Article  CAS  Google Scholar 

  10. P. C. H. Li and D. J. Harrison, Anal. Chem., 1997, 69, 1564.

    Article  CAS  Google Scholar 

  11. T. Tsuda, S. Kitagawa, and O. Nozaki, in Proceeding of the 10th International Symposium on HPCE, Kyoto, September 1997, 67.

    Google Scholar 

  12. T. Tsuda, S. Kitagawa, and O. Nozaki, in Proceeding of the 72th Spring Meeting of Chemical Society of Japan, Tokyo, March 1997, 104.

    Google Scholar 

  13. J. Janca, Tr. Anal. Chem., 1983, 2, 278.

    Article  CAS  Google Scholar 

  14. J. Janca, Tr. Anal. Chem., 1987, 6, 147.

    Article  CAS  Google Scholar 

  15. J. Yang, Y. Huang, X.-B. Wang, F. F. Becker, and P. R. C. Gascoyne, Anal. Chem., 1999, 71, 911.

    Article  CAS  Google Scholar 

  16. T. Tsuda, M. Ikedo, G. Jones, R. Dadoo, and R. N. Zare, J. Chromatogr., 1993, 632, 201.

    Article  CAS  Google Scholar 

  17. J. A. Taylor and E. S. Yeung, Anal. Chem., 1993, 65, 2928.

    Article  CAS  Google Scholar 

  18. T. Tsuda, S. Kitagawa, R. Dadoo, and R. N. Zare, Bunseki Kagaku, 1997, 46, 409.

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Applied Chemistry, Nagoya Institute of Technology, Gokiso, Showa, Nagoya, 466-8555, Japan

    Takao Tsuda, Norihiro Yamauchi & Shinya Kitagawa

Authors
  1. Takao Tsuda
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  2. Norihiro Yamauchi
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  3. Shinya Kitagawa
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Correspondence to Takao Tsuda.

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Tsuda, T., Yamauchi, N. & Kitagawa, S. Separation of Red Blood Cells at the Single Cell Level by Capillary Zone Electrophoresis. ANAL. SCI. 16, 847–850 (2000). https://doi.org/10.2116/analsci.16.847

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  • DOI: https://doi.org/10.2116/analsci.16.847

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