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Rapid Determination of Benzene Metabolites Phenol and p-Cresol in the Urine of Petrol Station Workers by Gas Chromatography

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Abstract

The determination of phenol and p-cresol in urine has been suggested as a suitable biomarker for the monitoring of benzene exposure of pump workers employed in petrol stations. A method for efficiently extracting a wide variety of drugs from urine for toxicological analysis by gas chromatography is presented. The sensitivity of the method is sufficient to monitor normal levels of phenol and p-cresol after exposure to benzene vapors. The detection limit of the method is low enough to measure urinary phenol and p-cresol at a concentration of 0.1 mg/l. The overall recoveries of urinary phenol and p-cresol relative to the internal standard, DPA, ranged between 95 and 96.5%. Our gas chromatographic method is a rapid, precise and economical one suitable to measure the metabolite benzene also in routine monitoring of phenol and p-cresol.

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Authors and Affiliations

  1. Department of Clinical Chemistry and Laboratory Medicine, University Surgical Clinic, Graz, Austria

    Gholam Ali Khoschsorur &  Walter Petek (head of Department of Clinical Chemistry and Laboratory Medicine)

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  1. Gholam Ali Khoschsorur
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  2. Walter Petek
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Correspondence to Gholam Ali Khoschsorur.

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Ali Khoschsorur, G., Petek, W. Rapid Determination of Benzene Metabolites Phenol and p-Cresol in the Urine of Petrol Station Workers by Gas Chromatography. ANAL. SCI. 16, 589–591 (2000). https://doi.org/10.2116/analsci.16.589

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  • DOI: https://doi.org/10.2116/analsci.16.589

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