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Piezoelectric Crystal Impedance Analysis for Investigating the Changes of Interfacial Properties due to Interaction of Cobalt Salt with DNA Immobilized on Biosensor

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Abstract

The interaction of a cobalt compound, tris(2,2′-bipyridyl)cobalt(III) perchlorate (Co(bpy)3(ClO4)3·3H2O), with DNA immobilized on a biosensor has been investigated by using a piezoelectric impedance technique. The changes of equivalent circuit parameters were used to characterize the changes of the interfacial properties of the piezoelectric biosensor in the reaction system. It was found that the interaction of cobalt compound with double-strained DNA (dsDNA) could be described by a sum of two exponential functions, suggesting two corresponding kinetic steps. As for dsDNA, the change of static capacitance showed a linear correlation with time. Comparative experiments indicated that dsDNA with double-helical structure is more easily bound with cobalt compound than single-strained DNA (ssDNA). From the frequency curve, some parameters related to this interaction, such as cross-linking ratio, binding ratio, and the maximum initial rate were roughly estimated to be 2%, 1.15, 0.72 Hz/s (or 0.12 Hz/s for ssDNA), respectively. The linear relationship between the frequency change and the motional resistance was verified to reflect the changes of the density-viscosity in the biological reaction.

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References

  1. A. H. Krotz, L. Y. Kuo, and J. K. Barton, Inorg. Chem., 1993, 32, 5963.

    Article  CAS  Google Scholar 

  2. B. A. Jackson and J. K. Barton, J. Am. Chem. Soc., 1997, 119, 12986.

    Article  CAS  Google Scholar 

  3. D. B. Hall, R. E. Holmin, and J. K. Barton, Nature. 1996, 382, 731.

    Article  CAS  Google Scholar 

  4. M. T. Carter, M. Rodriguez, and A. J. Bard, J. Am. Chem. Soc., 1989, 111, 8901.

    Article  CAS  Google Scholar 

  5. M. Rodriguez and A. J. Bard, Anal. Chem., 1990, 62, 2658.

    Article  CAS  Google Scholar 

  6. Y. D. Zhao, D. W. Pang, Z. L. Wang, J. K. Chang, and Y. P. Qi, J. Electroanal. Chem., 1997, 431, 203.

    Article  CAS  Google Scholar 

  7. X. H. Xu, H. C. Yang, T. E. Mallouk, and A. J. Bard, J. Am. Chem. Soc., 1994, 116, 8386.

    Article  CAS  Google Scholar 

  8. X. H. Xu and A. J. Bard, J. Am. Chem. Soc., 1995, 117, 2627.

    Article  CAS  Google Scholar 

  9. J. F. Alder and J. J. McCallum, Analyst. 1983, 108, 1169.

    Article  CAS  Google Scholar 

  10. M. R. Deakin and D. A. Buttry, Anal. Chem., 1989, 61, 1147.

    Article  Google Scholar 

  11. M. D. Ward and D. A. Buttry, Science. 1990, 249, 1000.

    Article  CAS  Google Scholar 

  12. M. Thompson, A. L. Kipling, W. C. Duncan-Hewitt, L. V. Rajakovic´, and B. A. Cˇavic´-Vlasak, Analyst. 1991, 116, 881.

    Article  CAS  Google Scholar 

  13. D. A. Buttry and M. D. Ward, Chem. Rev., 1992, 92, 1355.

    Article  CAS  Google Scholar 

  14. H. Muramatsu, Y. Watanabe, M. Hikuma, T. Ataka, I. Kubo, E. Tamiya, and I. Karube, Anal. Lett., 1989, 22, 2155.

    Article  Google Scholar 

  15. L. Deng, F. J. He, T. J. Jiang, L. H. Nie, and S. Z. Yao, J. Microbio. Methods. 1995, 23, 229.

    Article  Google Scholar 

  16. J. H. T. Luong, E. Prusak-Sochaczewki, and G. G. Guilbault, Ann. N. Y. Acad. Soc., 1990, 613, 439.

    Article  CAS  Google Scholar 

  17. H. Muramatsu, J. M. Dicks, E. Tamiya, and I. Karube, Anal. Chem., 1987, 59, 2760.

    Article  CAS  Google Scholar 

  18. E. S. Grabbe, R. P. Buck, and O. R. Melroy, J. Electroanal. Chem., 1987, 223, 67.

    Article  CAS  Google Scholar 

  19. S. Z. Yao, X. T. Zhang, and L. H. Nie, J. Pharm. Sci., 1991, 80, 17.

    Article  CAS  Google Scholar 

  20. L. H. Nie, X. T. Zhang, and S. Z. Yao, J. Pharm. Biomed. Anal., 1992, 10, 529.

    Article  CAS  Google Scholar 

  21. E. Tamiya, M. Gotoh, T. Matsu, M. Tanaka, and I. Karube, J. Mol. Catal., 1988, 43, 387.

    Article  Google Scholar 

  22. R. C. Ebersole and M. D. Ward, J. Am. Chem. Soc., 1988, 110, 8623.

    Article  CAS  Google Scholar 

  23. S. Yamaguchi, T. Shimomura, T. Tatsuma, and N. Oyama, Anal. Chem., 1993, 65, 1925.

    Article  CAS  Google Scholar 

  24. Y. Okahata, M. Kawase, K. Niikura, F. Ohtake, H. Furusawa, and Y. Ebara, Anal. Chem., 1998, 70, 1288.

    Article  CAS  Google Scholar 

  25. B. A. Cˇavic´ -Vlasak and L. J. V. Rajakovic´, Fresenius’ J. Anal. Chem., 1992, 343, 339.

    Article  Google Scholar 

  26. M. Yang, F. L. Chung, and M. Thompson, Anal. Chem., 1993, 65, 3713.

    Article  CAS  Google Scholar 

  27. G. Sauerbrey, Z. Phys., 1959, 155, 206.

    Article  CAS  Google Scholar 

  28. K. K. Kanazawa and J. G. Gordon, Anal. Chim. Acta. 1985, 177, 99.

    Article  Google Scholar 

  29. S. Z. Yao, “Piezoelectric Chem. & Biosensors”, 1997. Hunan Normal University Press, China, (in Chinese).

    Google Scholar 

  30. S. J. Matin, V. E. Grantaf, and G. C. Frye, Anal. Chem., 1991, 63, 2272.

    Article  Google Scholar 

  31. H. Muramatsu, E. Tamiya, and I. Karube, Anal. Chem., 1988, 60, 2142.

    Article  CAS  Google Scholar 

  32. T. A. Zhou, L. H. Nie, and S. Z. Yao, J. Electroanal. Chem., 1990, 293, 1.

    Article  CAS  Google Scholar 

  33. G. Hayward, Anal. Chim. Acta. 1992, 264, 23.

    Article  CAS  Google Scholar 

  34. V. E. Bottom, “Introduction to Crystal Unit Design”. 1982, Van Nostrand Reinhold, New York, 120.

    Google Scholar 

  35. M. Yang and M. Thompson, Anal. Chem.. 1993, 65, 3591.

    Article  CAS  Google Scholar 

  36. M. Yang, M. Thompson, and W. C. Duncan-Hewitt, Lagmuir. 1993, 9, 802.

    Article  CAS  Google Scholar 

  37. M. Yang and M. Thompson, Anal. Chem.. 1993, 65, 1158.

    Article  CAS  Google Scholar 

  38. F. H. Burstall and R. S. Nyholm, J. Chem. Soc. 1952, 3570.

    Google Scholar 

  39. M. A. Noël and P. A. Topart, Anal. Chem.. 1994, 66, 484.

    Article  Google Scholar 

  40. P. Bernabeu, L. Tamisier, A. D.E. Cesare, and A. Caprani, Electrochim. Acta. 1988, 33, 1129.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China

    Anhong Zhou, Lihua Nie & Shouzhuo Yao

  2. Chemical Research Institute, Hunan Normal University, Changsha, 410081, P. R. China

    Qingji Xie & Youyu Zhang

Authors
  1. Anhong Zhou
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  2. Qingji Xie
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  3. Youyu Zhang
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  4. Lihua Nie
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  5. Shouzhuo Yao
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Correspondence to Shouzhuo Yao.

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Zhou, A., Xie, Q., Zhang, Y. et al. Piezoelectric Crystal Impedance Analysis for Investigating the Changes of Interfacial Properties due to Interaction of Cobalt Salt with DNA Immobilized on Biosensor. ANAL. SCI. 16, 467–472 (2000). https://doi.org/10.2116/analsci.16.467

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  • DOI: https://doi.org/10.2116/analsci.16.467

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