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Peroxidase-like Activity of Glass-Beads Modified with Metal-Porphines and Their Analytical Applications

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Abstract

Peroxidase-like activities of glass-beads modified with metal-porphines (M-PG) were examined through a dye-formation reaction, which was catalyzed by peroxidase, of phenol and 4-aminoantipyrine in the presence of hydrogen peroxide. When M-PG was used as a catalyst in place of peroxidase, glass-beads modified with Mn3+- and Fe3+-tetrakis(4-carboxyphenyl)porphine derivatives (Mn- and Fe-TCPPG) showed relatively strong peroxidase-like activity among the M-PG examined. The percentage of the resulting quinoid dye by Mn- and Fe-TCPPG reached to about 93% and 88%, respectively, of that formed by peroxidase. Peroxidase-like activity of Mn-TCPPG was applied to the determination of hydrogen peroxide in place of peroxidase. Furthermore, the determination of serum compounds (glucose and uric acid), which form hydrogen peroxide by using their oxidases, was examined.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama, 700-8530, Japan

    Masaki Mifune, Katsuyoshi Sugimoto & Yutaka Saito

  2. Graduate School of Sciences and Technology, Okayama University, Tsushima-Naka, Okayama, 700-8530, Japan

    Akimasa Iwado & Hiromichi Akizawa

Authors
  1. Masaki Mifune
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  2. Akimasa Iwado
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  3. Hiromichi Akizawa
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  4. Katsuyoshi Sugimoto
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  5. Yutaka Saito
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Mifune, M., Iwado, A., Akizawa, H. et al. Peroxidase-like Activity of Glass-Beads Modified with Metal-Porphines and Their Analytical Applications. ANAL. SCI. 16, 1121–1125 (2000). https://doi.org/10.2116/analsci.16.1121

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  • DOI: https://doi.org/10.2116/analsci.16.1121

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