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Usefulness of Derivatization in High-Performance Liquid Chromatography/Tandem Mass Spectrometry of Conjugated Vitamin D Metabolites

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Abstract

The usefulness of derivatization in high-performance liquid chromatography/ion trap tandem mass spectrometry (electro-spray ionization) of conjugated vitamin D metabolites was examined. 24,25-Dihydroxyvitamin D3 [24,25(OH)2D3]-3-and -24-glucuronides (G) were converted to the adducts with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), the methyl esters and the acetates. The combination of the derivatization with PTAD and methylation improved the detection limit in both the positive- and negative-ion modes. The PTAD adducts of 24,25(OH)2D3-3G and -24G also gave a characteristic product ion indicating the conjugation position by the positive-MS/MS/MS, which was not observed in the intact forms. 25-Hydroxyvitamin D3 3-sulfate gave the deprotonated ion in the negative-ion mode, but no characteristic ion was observed in the positive-ion mode. On the contrary, its PTAD adduct gave a molecular-related ion in the positive-ion mode, the MS/MS/MS of which provided structural information, that is, the conjugation position and the existence of a sulfuric acid group.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-0934, Japan

    Tatsuya Higashi, Kanako Miura, Junichi Kitahori & Kazutake Shimada

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  1. Tatsuya Higashi
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  2. Kanako Miura
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  3. Junichi Kitahori
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  4. Kazutake Shimada
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Higashi, T., Miura, K., Kitahori, J. et al. Usefulness of Derivatization in High-Performance Liquid Chromatography/Tandem Mass Spectrometry of Conjugated Vitamin D Metabolites. ANAL. SCI. 15, 619–623 (1999). https://doi.org/10.2116/analsci.15.619

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  • DOI: https://doi.org/10.2116/analsci.15.619

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