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Highly Sensitive Electrochemiluminescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads

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Abstract

The newer electrochemiluminescence (ECL) immunoassay system was established by using both an antibody coated on paramagnetic micro-beads (Capt-MB) as a carrier of the immunoassay and an antibody labeled with ruthenium(II) tris-bipyridine-NHS (Ru-Ab). The ECL excitations were designed to be generated upon the surface of the working electrode which collected the reacted Capt-MB by magnetic force. As a model of the immunoassay, the reaction between alpha-fetoprotein (AFP) and anti-AFP antibodies was used in accordance with the so-called sandwich method, where the sand-wich conformation of AFP-(Ru-Ab) was made on the surface of the Capt-MB. The ECL immunoassay system revealed the following results: a) the ECL signal intensity was obtained in proportion to the AFP concentration of each specimen; b) the dynamic range of this ECL immunoassay system was extended to 10000 times of magnitude in the 2-step assay; and c) the detection sensitivity reached to the level of 5 pg/ml in the AFP concentration after 15 min in the 1-step immunoassay.

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Authors and Affiliations

  1. Clinical Diagnostics Department, Eisai Co., Ltd., 5-5-5 Koishikawa, Bunkyo, Tokyo, 112-8088, Japan

    Yuzaburo Namba & Michinori Usami

  2. Department of Fermentation Technology, Hiroshima University, Kagamiyama, Higashi-Hiroshima, 739-8527, Japan

    Osamu Suzuki

Authors
  1. Yuzaburo Namba
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  2. Michinori Usami
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  3. Osamu Suzuki
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Namba, Y., Usami, M. & Suzuki, O. Highly Sensitive Electrochemiluminescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads. ANAL. SCI. 15, 1087–1093 (1999). https://doi.org/10.2116/analsci.15.1087

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  • DOI: https://doi.org/10.2116/analsci.15.1087

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