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Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell

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An Erratum to this article was published on 11 December 1998

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Abstract

The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.

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Authors and Affiliations

  1. Department of Molecular Science and Technology, Kyushu University, Kasuga-shi, Fukuoka, 816-8580, Japan

    Joon Myong Song & Takanori Inoue

  2. Department of Industrial Chemistry, Kinki University in Kyushu, Iizuka 820, Japan

    Joon Myong Song & Hirofumi Kawazumi

  3. Department of Chemistry, Iowa State University, Ames, IA, 50011, USA

    Takanori Inoue

  4. Department of Applied Chemistry, Oita University, Tannohara, Oita, 845, Japan

    Teiichiro Ogawa

  5. Department of Molecular and Material Sciences, Kyushu University, Kasuga-shi, Fukuoka, 816-8580, Japan

    Teiichiro Ogawa

Authors
  1. Joon Myong Song
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  2. Takanori Inoue
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  3. Hirofumi Kawazumi
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  4. Teiichiro Ogawa
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Song, J.M., Inoue, T., Kawazumi, H. et al. Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell. ANAL. SCI. 14, 913–916 (1998). https://doi.org/10.2116/analsci.14.913

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  • DOI: https://doi.org/10.2116/analsci.14.913

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