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High-Performance Liquid-Chromatographic Determination of Guanosine 3′,5′-Cyclic Monophosphate in Human Urine with Fluorescence Detection Using 3,4-Dimethoxyphenylglyoxal

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Abstract

A sensitive and selective method for the determination of guanosine 3′,5′-cyclic monophosphate in human urine is described. The method uses high-performance liquid chromatography with fluorescence detection. Guanosine 3′,5′-cyclic monophosphate and other guanine nucleotides in human urine, after a clean-up with an anion exchanger cartridge (TOYOPAK DEAES), gave fluorescence products by a reaction with 3,4-dimethoxyphenylglyoxal in a neutral medium. The formed derivatives were separated using reversed-phase high-performance liquid chromatography. The limit in the detection (S/N=3) of guanosine 3′,5′-cyclic monophosphate is 4.4 pmol on the column.

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Author notes

  1. Yoshihito Ohba

    Present address: Dojindo Laboratories, Tabaru 2025-5, Mashiki-machi, Kamimashiki-gun, Kumamoto, 861-23, Japan

Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kyushu University 62, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

    Yoshihito Ohba, Masaaki Kai, Yosuke Ohkura & Kiyoshi Zaitsu

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  1. Yoshihito Ohba
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  2. Masaaki Kai
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  3. Yosuke Ohkura
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  4. Kiyoshi Zaitsu
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Ohba, Y., Kai, M., Ohkura, Y. et al. High-Performance Liquid-Chromatographic Determination of Guanosine 3′,5′-Cyclic Monophosphate in Human Urine with Fluorescence Detection Using 3,4-Dimethoxyphenylglyoxal. ANAL. SCI. 13, 469–472 (1997). https://doi.org/10.2116/analsci.13.469

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  • DOI: https://doi.org/10.2116/analsci.13.469

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