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Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to the Urine of a Patient with Zellweger Syndrome

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Abstract

Gas chromatographic determination of C27-bile acids was investigated for the study of bile acid biosynthesis and related congenital disorders. The intermediate C27-bile acids in the β-oxidation step and related compounds were conveniently synthesized from the corresponding C24-bile acids as standard samples. The separation of methyl ester-trimethylsilyl ether (Me-TMS) and methyl ester-dimethylethylsilyl ether (Me-DMES) derivatives of the C27-bile acids was carried out by capillary gas chromatography. Then methylene units of the above derivatives were measured. The Me-DMES derivatives were more efficient for the separation of C:7-bile acids than the Me-TMS derivatives. The calibration curves for the Me-DMES derivatives of C27-bile acids showed good linearities over the range of 5 – 200 ng; the detection limits were ca. 0.5 ng (S/N=5). The relative recoveries from biological samples were 93 – 105% for all C27-bile acids using 24-nor-chenodeoxycholic acid as an internal standard. An application to the diagnosis of Zellweger syndrome is also discussed.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, 061-02, Japan

    Takao Kurosawa, Masahiro Sato, Fumihiko Kikuchi & Masahiko Tohma

  2. Department of Pediatrics, Akita University School of Medicine, Akita, 010, Japan

    Yusaku Tazawa

Authors
  1. Takao Kurosawa
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  2. Masahiro Sato
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  3. Fumihiko Kikuchi
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  4. Yusaku Tazawa
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  5. Masahiko Tohma
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Kurosawa, T., Sato, M., Kikuchi, F. et al. Capillary Gas Chromatographic Determination of C27-Bile Acids in Biological Samples and Its Application to the Urine of a Patient with Zellweger Syndrome. ANAL. SCI. 12, 839–846 (1996). https://doi.org/10.2116/analsci.12.839

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  • DOI: https://doi.org/10.2116/analsci.12.839

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