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Post-Electrophoresis Capillary Scanner for Detecting DNA Sequencing Reaction Products

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Abstract

A post-electrophoresis capillary scanning technique has been developed and its feasibility as a high-throughput DNA sequencing method has been demonstrated. In this method, capillary gel electrophoresis separations of DNA sequencing reaction products are spatially and temporally separated with laser-induced fluorescence detection of the products. A 90-cm gel-filled capillary was used to separate a mixture of the fluorescently labeled adenine-terminated DNA fragments of pBluescript SK(-) DNA. The adenine base sequence of the DNA was then determined up to 350 bases with a scan rate of 3.7 cm/s, which took 27 s for the capillary. We have estimated that the capillary scanner can analyze over 130 capillaries in 1 h, which corresponds to a DNA sequencing throughput of about 1 megabase/day.

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Authors and Affiliations

  1. Department of Chemistry, Pohang University of Science and Technology, San 31 Hyoja Dong, Pohang 790-784, South Korea

    Suhyeon Kim, Hyun Ju Yoo & Jong Hoon Hahn

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  1. Suhyeon Kim
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  2. Hyun Ju Yoo
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  3. Jong Hoon Hahn
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Kim, S., Yoo, H.J. & Hahn, J.H. Post-Electrophoresis Capillary Scanner for Detecting DNA Sequencing Reaction Products. ANAL. SCI. 12, 371–375 (1996). https://doi.org/10.2116/analsci.12.371

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  • DOI: https://doi.org/10.2116/analsci.12.371

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