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Coulometric Detection of Peptides by Reversed-Phase Liquid Chromatography Using an In-Line Reactor Containing Copper Metal

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Abstract

A general method has been developed for the selective coulometric detection of peptides using reversed-phase liquid chromatography. This method is based on the formation of electrochemically oxidizable copper(II)-peptide complexes by the reaction of peptides with copper(II) ions in an alkaline mobile phase through an in-line reactor containing copper metal. Di-, tri- and tetrapeptides used as model compounds (6 species) are separated on a column containing CIS-bonded vinyl alcohol copolymer gel (Asahipak ODP-50,5 μm, 150 mm×6 mm i.d.) and an alkaline mobile phase (pH 11.0, 25 mM phosphate buffer). A detection limit of 3 ng was obtained for alanylalanylalanylalanine.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Kitasato University, Shirokane, Minato, Tokyo, 108, Japan

    Hiroaki Kubo, Akiko Tsujimura & Toshio Kinoshita

Authors
  1. Hiroaki Kubo
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  2. Akiko Tsujimura
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  3. Toshio Kinoshita
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Kubo, H., Tsujimura, A. & Kinoshita, T. Coulometric Detection of Peptides by Reversed-Phase Liquid Chromatography Using an In-Line Reactor Containing Copper Metal. ANAL. SCI. 10, 579–582 (1994). https://doi.org/10.2116/analsci.10.579

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  • DOI: https://doi.org/10.2116/analsci.10.579

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