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Determination of Glucose Oxidase by Means of the Chemiluminescence of Cypridina Luciferin Analogue

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Abstract

FeCl2 was found to be a useful catalyst for the determination of H2O2 based on the chemiluminescence of a Cypridina luciferin analogue, 2-methyl-6-phenyl-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA). By this method, H2O2 was detected in a range from 300 pmol/ml to 10 nmol/ml. In addition, glucose oxidase (GOD) was determined in a range from 1.18×10−5 units/ml (280 amol/ml) to 7.56×10−4 units/ml (17.9 fmol/ml) when GOD was incubated with glucose for 10 min at 34°C.

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References

  1. K. Puget, A. M. Michelson and S. Avrameas, Anal. Biochem., 79, 447 (1977).

    Article  CAS  Google Scholar 

  2. M. Tabata, C. Fukunaga, M. Ohyabu and T. Murachi, J. Appl. Biochem., 6, 251 (1984).

    CAS  PubMed  Google Scholar 

  3. D. T. Bostick and D. M. Hercules, Anal. Lett., 7, 347 (1974).

    Article  CAS  Google Scholar 

  4. H. Arakawa, M. Maeda and A. Tsuji, Chem. Pharm. Bull, 30, 3036 (1982).

    Article  CAS  Google Scholar 

  5. H. Arakawa, M. Maeda and A. Tsuji, Clin. Chem., 31, 430 (1985).

    Article  CAS  Google Scholar 

  6. H. Sawada, K. Masuyama, M. Mitani and M. Nakayama, Bunseki Kagaku, 39, 573 (1990).

    Article  CAS  Google Scholar 

  7. A. Tsuji, M. Maeda and H. Arakawa in “Kouso men-eki sokuteihou (Enzyme Immunoassay, in Japanese)”, ed. E. Ishikawa, T. Kawai and K. Miyai, p.71, Igakushoin, Tokyo, 1987.

  8. T. Goto, Proc. Inti. Symp. on Activation of Dioxygen and Homogeneous Catalytic Oxidations, Studies in Organic Chemistry, Vol. 33, p. 367, 1987.

    Google Scholar 

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Authors and Affiliations

  1. Tsukuba Research Laboratory, NOF Corporation, Tsukuba, Ibaraki, 300-26, Japan

    Motohiro Mitani & Yasumi Koinuma

  2. Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376, Japan

    Masanori Kosugi

Authors
  1. Motohiro Mitani
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  2. Yasumi Koinuma
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  3. Masanori Kosugi
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Mitani, M., Koinuma, Y. & Kosugi, M. Determination of Glucose Oxidase by Means of the Chemiluminescence of Cypridina Luciferin Analogue. ANAL. SCI. 10, 267–269 (1994). https://doi.org/10.2116/analsci.10.267

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  • DOI: https://doi.org/10.2116/analsci.10.267

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