Abstract
A high performance liquid chromatographic( HPLC) determination of pyrrolidine, piperidine, and morpholine in water at μg/l level has been developed. These heterocyclic amines are converted to their 1,2-naphthoquinone derivatives by pre-column derivatization with potassium 1,2-naphthoquinone-4-sulfonate. The derivatives are extracted into dichloromethane, concentrated, and determined by a HPLC system equipped with a photometer set at 436 nm. This proposed method is simple, sensitive, and selective for detection of trace amounts of amines in water. The calibration curves of amines were linear up to 100 μg/l. The precision of the method is 0.9 to 4,7% for the analysis of water at the 10 μg/l level. By this method, piperidine was detected at 0.4 xg/l in an effluent of a biological wastewater treatment plant.
Similar content being viewed by others
References
R. Montesano and H. Bartsch, Mutation Res., 32, 179 (1976).
A. D. Corria, R. Samperi and C. Severini, J. Chromatogr., 170, 325 (1979).
K. Kuwata, Y. Yamazaki and M. Uebori, Bunseki Kagaku, 29, 170 (1980).
J. K. Lin and C. C. Lai, Anal. Chem., 52, 630 (1980).
Y. Suzuki and R. Miyagawa, Bunseki Kagaku, 30, 81 (1981).
E. W. Day, Jr., T. Golab and J. R. Koons, Anal. Chem., 38, 1053 (1966).
M. Koga, T. Akiyama and R. Shinohara, Bunseki Kagaku, 30, 745 (1981).
D. H. Karweik and C. H. Meyers, Anal. Chem., 51, 319 (1979).
C. R. Clark, C. M. Darling, J. L. Chan and A. C. Nichols, Anal. Chem., 49, 2080 (1977).
O. Folin, J. Biol. Chem., 51, 377 (1922).
D. H. Rosenblatt, P. Hlinka and J. Epstein, Anal. Chem., 27, 1290 (1955).
M. Pesez and J. Bartos, Ann. Pharm. Fr., 27, 161 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koga, M., Akiyama, T. Determination of Trace Heterocyclic Amines in Water as Their 1,2-Naphthoquinone Derivatives by High Performance Liquid Chromatography. ANAL. SCI. 1, 285–288 (1985). https://doi.org/10.2116/analsci.1.285
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.1.285