Article PDF
Avoid common mistakes on your manuscript.
References
Hayashi S, Kaminaga T, Baba A, et al. Diagnostic value of a nested polymerase chain reaction for diagnosing cutaneous sporotrichosis from paraffin-embedded skin tissue. Mycoses 2019; 62: 1148–53.
de Meyer EM, de Beer ZW, Summerbell RC, et al. Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia 2008; 100: 647–61.
De Lima Barros MB, de Almeida Paes R, Schubach AO. Sporothrix schenckii and Sporotrichosis. Clin Microbiol Rev 2011; 24: 633–54.
Arenas R, Sánchez-Cardenas CD, Ramirez-Hobak L, Ruíz Arriaga LF, Vega Memije ME. Sporotrichosis: from KOH to Molecular Biology. J Fungi (Basel) 2018; 4: 62.
Rodríguez-Brito S, Camacho E, Mendoza M, Niño-Vega GA. Differential identification of Sporothrix spp. and Leishmania spp. by conventional PC and qPCR in multiplex format. Med Mycol 2015; 53: 22–7.
Hu S, Chung WH, Hung S, et al. Detection of Sporothrix schenckii in clinical samples by a nested PCR assay. J Clin Microbiol 2003; 41: 1414–8.
Zhang M, Li F, Li R, Gong J, Zhao F. Fast diagnosis of sporotrichosis caused by Sporothrix globosa, Sporothrix schenckii, and Sporothrix brasiliensis based on multiplex real-time PCR. PLoS Negl Trop Dis 2019; 13: e0007219.
Author information
Authors and Affiliations
Corresponding author
Additional information
Disclosure
Conflicts of interest: none. Acknowledgements: this study was supported by a grant from Dokkyo Medical University, Young Investigator Award (No.2019-10), Tochigi, Japan. Ethical Statement: informed consent was obtained from the study participants, including consent to participate and publish the findings. The authors confirm that the ethical policies of the journal, as noted in the Declaration of Helsinki, have been adhered to and appropriate ethical review and approval by the institutional review board of Dokkyo Medical University were received.
About this article
Cite this article
Koike, M., Hayashi, S., Baba, A. et al. PCR-based diagnosis of Sporothrix infection using DNA from paraffin-embedded skin specimens in previously undiagnosed cases. Eur J Dermatol 30, 614–615 (2020). https://doi.org/10.1684/ejd.2020.3862
Published:
Issue Date:
DOI: https://doi.org/10.1684/ejd.2020.3862