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Serum miR-124 up-regulation as a disease marker of toxic epidermal necrolysis

  • Investigative Report
  • Published:
European Journal of Dermatology Aims and scope

Abstract

Background

Toxic epidermal necrolysis (TEN) is a lethal complication of drugs, thus early diagnosis and treatment are important. However, there are no satisfactory clinical biomarkers of TEN.

Objectives

We investigated miR-124 and miR-214 expressions in serum and skin tissues of severe drug eruptions to evaluate the possibility of biomarkers of TEN.

Materials & Methods

microRNAs were extracted from serum and skin tissues. Serum samples were obtained from 7 TEN patients, 5 Stevens-Johnson syndrome (SJS) patients, 11 erythema multiforme (EM) minor patients and 21 healthy volunteers. Skin tissues were obtained from 4 TEN patients, 3 SJS patients, 8 EM minor patients, 3 psoriasis and 3 atopic dermatitis patients. Six control skin samples were obtained. MicroRNA concentrations were determined by PCR array and real-time PCR.

Results

The concentrations of miR-124 in sera from TEN were significantly higher than those from healthy controls. In the characteristics curve analysis of serum miR-124 for differentiating TEN patients from normal subjects, the area under curve was 0.94. The serum miR-124 concentration was strongly correlated with the erosion area and the SCORTENscale. The expression of miR-214was significantly increased in the skin of TEN.

Conclusion

The serum miR-124 concentration can be used as a disease activity marker for severe drug eruptions, reflecting the severity of keratinocyte apoptosis.

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Authors and Affiliations

  1. Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, 860-8556, Japan

    Shoki Sato, Asako Ichihara, Masatoshi Jinnin, Yuki Izuno, Satoshi Fukushima & Hironobu Ihn

Authors
  1. Shoki Sato
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  2. Asako Ichihara
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  3. Masatoshi Jinnin
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  4. Yuki Izuno
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  5. Satoshi Fukushima
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  6. Hironobu Ihn
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Corresponding author

Correspondence to Asako Ichihara.

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Sato, S., Ichihara, A., Jinnin, M. et al. Serum miR-124 up-regulation as a disease marker of toxic epidermal necrolysis. Eur J Dermatol 25, 457–462 (2015). https://doi.org/10.1684/ejd.2015.2621

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  • DOI: https://doi.org/10.1684/ejd.2015.2621

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