European Cytokine Network

, Volume 27, Issue 4, pp 102–107 | Cite as

Direct effects of interleukin-7 on the function of human T cells in vitro

  • Vyacheslav A. Shmarov
  • Vladimir V. Malashchenko
  • Maksim E. Meniailo
  • Natalia D. Gazatova
  • Natalia M. Todosenko
  • Olga B. Melashchenko
  • Andrei G. Goncharov
  • Victor I. Seledtsov
Research Article
  • 24 Downloads

Abstract

CD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in CD4-positive and in CD4-negative effector memory (CD45RA-CD197-) T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) T cells, without significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+), and effector memory (CD45RA-CD197-) T-cell compartments. In addition, IL-7 facilitated activation of CD4- (but not CD4+) terminally differentiated effector (CD45RA+CD197-) T cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets.

Key words

interleukin-7 T cell homeostatic activity T-cell activation CD25 cytokine production 

Abbreviations

Ab

antibody

IFN

interferon

IL

interleukin

PBMC

peripheral blood mononuclear cell

Th

helper T cell

Treg

regulatory T cell

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Copyright information

© John Libbey Eurotext 2016

Authors and Affiliations

  • Vyacheslav A. Shmarov
    • 1
  • Vladimir V. Malashchenko
    • 1
  • Maksim E. Meniailo
    • 1
  • Natalia D. Gazatova
    • 1
  • Natalia M. Todosenko
    • 1
  • Olga B. Melashchenko
    • 1
  • Andrei G. Goncharov
    • 1
  • Victor I. Seledtsov
    • 1
    • 2
  1. 1.Immanuel Kant Baltic Federal UniversityKaliningradRussia
  2. 2.Immanuel Kant Baltic Federal UniversityKaliningradRussia

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