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European Cytokine Network

, Volume 25, Issue 1, pp 14–23 | Cite as

Whole-body cryostimulation as an effective way of reducing exercise-induced inflammation and blood cholesterol in young men

  • Ewa ZiemannEmail author
  • Robert A. Olek
  • Tomasz Grzywacz
  • Jan J. Kaczor
  • Jędrzej Antosiewicz
  • Wojciech Skrobot
  • Sylwester Kujach
  • Radosław Laskowski
Research Article

Abstract

Inflammation may accompany obesity and a variety of diseases, or result from excessive exercise. The aim of this study was to investigate the anti-inflammatory effect of whole-body cryostimulation on the inflammatory response induced by eccentric exercise under laboratory conditions. The study also sought to establish if cold treatment changes the lipid profile and modifies energy expenditure in young people. Eighteen healthy and physically active, college-aged men volunteered to participate in the experiment. They were divided into two subgroups: CRYsubmitted to whole-body cryostimulation, and CONT- a control group. Both groups performed eccentric work to induce muscle damage. Blood samples were collected before and 24 h after the exercise. Over the five days that followed, the CRY group was exposed to a series of 10 sessions in a cryogenic chamber (twice a day, for 3 min, at a temperature of −110°C). After this period of rest, both groups repeated a similar eccentric work session, following the same schedule of blood collection. The perceived pain was noted 24h after each session of eccentric workout. A 30-minute step up/down work-out induced delayed-onset muscle soreness in both groups. The five-day recovery period accompanied by exposure to cold significantly enhanced the concentration of the anti-inflammatory cytokine IL-10. It also led to a pronounced reduction in levels of the pro-inflammatory cytokine IL-1β, and reduced muscle damage. The values for IL-10 before the second bout of eccentric exercise in the CRY group were 2.0-fold higher in comparison to baseline, whereas in the CONT group, the concentration remained unchanged. Furthermore, blood concentrations of the pro-inflammatory cytokine IL-1β fell significantly in the CRY group. The main finding of this study was that a series of 10 sessions of whole body cryostimulation significantly reduced the inflammatory response induced by eccentric exercise. The lipid profile was also improved, but there was no effect on energy expenditure during the exercise.

Key words

muscle damage cytokines cold air exposure 

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References

  1. 1.
    Blair SN. Physical inactivity: the biggest public health problem of the 21st century. Br J Sports Med 2009; 43: 1–2.PubMedGoogle Scholar
  2. 2.
    Blair SN, Morris JN. Healthy hearts-and the universal benefits of being physically active: physical activity and health. Ann Epidemiol 2009; 19: 253–6.PubMedCrossRefGoogle Scholar
  3. 3.
    Lee IM, Shiroma EJ, Lobelo F, et al. Effect of physical inactivity on major non-communicable diseases worldwide: an analysis of burden of disease and life expectancy. Lancet 2012; 380: 219–29.PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Pedersen BK. The anti-inflammatory effect of exercise: its role in diabetes and cardiovascular disease control. Essays Biochem 2006; 42: 105–17.PubMedCrossRefGoogle Scholar
  5. 5.
    Tidball JG. Inflammatory processes in muscle injury and repair. Am J Physiol Regul Integr Comp Physiol 2005; 288: R345–53.CrossRefGoogle Scholar
  6. 6.
    Paulsen G, Mikkelsen UR, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev 2012; 18: 42–97.PubMedGoogle Scholar
  7. 7.
    Braun WA, Dutto DJ. The effects of a single bout of downhill running and ensuing delayed onset of muscle soreness on running economy performed 48 h later. Eur J Appl Physiol 2003; 90: 29–34.PubMedCrossRefGoogle Scholar
  8. 8.
    Clarkson PM, Hubal MJ. Exercise-induced muscle damage in humans. Am J Phys Med Rehabil 2002; 81: S52–69.CrossRefGoogle Scholar
  9. 9.
    Aoi W, Naito Y, Yoshikawa T. Role of oxidative stress in impaired insulin signaling associated with exercise-induced muscle damage. Free Radic Biol Med 2013; 65: 1265–72.PubMedCrossRefGoogle Scholar
  10. 10.
    Costello JT, Algar LA, Donnelly AE. Effects of whole-body cryotherapy (-110 degrees C) on proprioception and indices of muscle damage. Scand J Med Sci Sports 2012; 22: 190–8.PubMedCrossRefGoogle Scholar
  11. 11.
    Hausswirth C, Louis J, Bieuzen F, et al. Effects of whole-body cryotherapy vs. far-infrared vs. passive modalities on recovery from exercise-induced muscle damage in highly-trained runners. PLoS One 2011; 6: e277–9.CrossRefGoogle Scholar
  12. 12.
    Costello JT, Baker PRA, Minett GM, et al. Whole-body cryotherapy (extreme cold air exposure) for preventing and treating muscle soreness after exercise in adults (Protocol). Cochrane Database of Systematic Reviews 2013.Google Scholar
  13. 13.
    Torres R, Ribeiro F, Alberto Duarte J, Cabri JM. Evidence of the physiotherapeutic interventions used currently after exercise-induced muscle damage: Systematic review and meta-analysis. Phys Ther Sport 2012; 13: 101–14.PubMedCrossRefGoogle Scholar
  14. 14.
    Barnett A. Using recovery modalities between training sessions in elite athletes: does it help? Sports Med 2006; 36: 781–96.PubMedCrossRefGoogle Scholar
  15. 15.
    Banfi G, Lombardi G, Colombini A, Melegati G. Whole-body cryotherapy in athletes. Sports Med 2010; 40: 509–17.PubMedCrossRefGoogle Scholar
  16. 16.
    Lubkowska A, Szygula Z, Chlubek D, Banfi G. The effect of prolonged whole-body cryostimulation treatment with different amounts of sessions on chosen pro- and anti-inflammatory cytokines levels in healthy men. Scand J Clin Lab Invest 2011; 71: 419–25.PubMedCrossRefGoogle Scholar
  17. 17.
    Ziemann E, Olek RA, Kujach S, et al. Five-day whole-body cryostimulation, blood inflammatory markers, and performance in high-ranking professional tennis players. J Athl Train 2012; 47: 664–72.PubMedCentralPubMedCrossRefGoogle Scholar
  18. 18.
    Di Renzo L, Carbonelli MG, Bianchi A, et al. Body composition changes after laparoscopic adjustable gastric banding: what is the role of-174G>C interleukin-6 promoter gene polymorphism in the therapeutic strategy? Int J Obes (Lond) 2012; 36: 369–78.CrossRefGoogle Scholar
  19. 19.
    Costello JT, Culligan K, Selfe J, Donnelly AE. Muscle, skin and core temperature after-110 degrees c cold air and 8 degrees c water treatment. PLoS One 2012; 7: e48190.CrossRefGoogle Scholar
  20. 20.
    Lubkowska A, Banfi G, Dolegowska B, et al. Changes in lipid profile in response to three different protocols of whole-body cryostimulation treatments. Cryobiology 2010; 61: 22–6.PubMedCrossRefGoogle Scholar
  21. 21.
    Ziemann E, Grzywacz T, Luszczyk M, et al. Aerobic and anaerobic changes with high-intensity interval training in active college-aged men. J Strength Cond Res 2011; 25: 1104–12.PubMedCrossRefGoogle Scholar
  22. 22.
    Newham DJ, Jones DA, Edwards RH. Large delayed plasma creatine kinase changes after stepping exercise. Muscle Nerve 1983; 6: 380–5.PubMedCrossRefGoogle Scholar
  23. 23.
    Szczesna-Kaczmarek A. Muscle fiber injury after eccentric exercise. Acta Biochimica Polonica 2007; 54: 1–5.Google Scholar
  24. 24.
    Jeukendrup AE, Wallis GA. Measurement of substrate oxidation during exercise by means of gas exchange measurements. Int J Sports Med 2005; 26 Suppl 1: S28–37.CrossRefGoogle Scholar
  25. 25.
    Flandry F, Hunt JP, Terry GC, Hughston JC. Analysis of subjective knee complaints using visual analog scales. Am J Sports Med 1991; 19: 112–8.PubMedCrossRefGoogle Scholar
  26. 26.
    Gulick DT, Kimura IF, Sitler M, Paolone A, Kelly JD. Various treatment techniques on signs and symptoms of delayed onset muscle soreness. J Athl Train 1996; 31: 145–52.PubMedCentralPubMedGoogle Scholar
  27. 27.
    Lubkowska A, Szygula Z, Klimek AJ, Torii M. Do sessions of cryostimulation have influence on white blood cell count, level of IL6 and total oxidative and antioxidative status in healthy men? Eur J Appl Physiol 2010; 109: 67–72.PubMedCrossRefGoogle Scholar
  28. 28.
    Garaulet M, Ordovas JM, Madrid JA. The chronobiology, etiology and pathophysiology of obesity. Int J Obes (Lond) 2010; 34: 1667–83.CrossRefGoogle Scholar
  29. 29.
    Rao SR. Inflammatory markers and bariatric surgery: a metaanalysis. Inflamm Res 2012; 61: 789–807.PubMedCrossRefGoogle Scholar
  30. 30.
    Sood A. Obesity, adipokines, and lung disease. J Appl Physiol (1985) 2010; 108: 744–53.CrossRefGoogle Scholar
  31. 31.
    Cooper DM, Radom-Aizik S, Schwindt C, Zaldivar Jr. F. Dangerous exercise: lessons learned from dysregulated inflammatory responses to physical activity. J Appl Physiol 2007; 103: 700–9.PubMedCrossRefGoogle Scholar
  32. 32.
    Smith LL. Cytokine hypothesis of overtraining: a physiological adaptation to excessive stress? Med Sci Sports Exerc 2000; 32: 317–31.PubMedCrossRefGoogle Scholar
  33. 33.
    Ziemann E, Zembron-Lacny A, Kasperska A, et al. Exercise traininginduced changes in inflammatory mediators and heat shock proteins in young tennis players. J Sports Sci Med 2013; 12: 282–9.PubMedCentralPubMedGoogle Scholar
  34. 34.
    Mucci P, Durand F, Lebel B, Bousquet J, Prefaut C. Interleukins 1- beta,-8, and histamine increases in highly trained, exercising athletes. Med Sci Sports Exerc 2000; 32: 1094–100.PubMedCrossRefGoogle Scholar
  35. 35.
    Nieman DC, Henson DA, Smith LL, et al. Cytokine changes after a marathon race. J Appl Physiol 2001; 91: 109–14.PubMedGoogle Scholar
  36. 36.
    Ostrowski K, Rohde T, Zacho M, Asp S, Pedersen BK. Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running. J Physiol 1998; 508(Pt 3): 949–53.PubMedCentralPubMedCrossRefGoogle Scholar
  37. 37.
    Suzuki K, Nakaji S, Yamada M, et al. Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc Immunol Rev 2002; 8: 6–48.PubMedGoogle Scholar
  38. 38.
    Malm C, Nyberg P, Engstrom M, et al. Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies. J Physiol 2000; 529 Pt 1: 243–62.PubMedCentralPubMedCrossRefGoogle Scholar
  39. 39.
    Nosaka K, Sakamoto K, Newton M, Sacco P. How long does the protective effect on eccentric exercise-induced muscle damage last? Med Sci Sports Exerc 2001; 33: 1490–5.PubMedCrossRefGoogle Scholar
  40. 40.
    Banfi G., Barassi A. et al. Effects of whole-body cryotherapy on serum mediators of inflammation and serum muscle enzymes in athletes. J Therm Biol 2009; 34: 55–9.CrossRefGoogle Scholar
  41. 41.
    Hirvonen HE, Mikkelsson MK, Kautiainen H, Pohjolainen TH, Leirisalo-Repo M. Effectiveness of different cryotherapies on pain and disease activity in active rheumatoid arthritis. A randomised single blinded controlled trial. Clin Exp Rheumatol 2006; 24: 295–301.PubMedGoogle Scholar
  42. 42.
    Wilcock IM, Cronin JB, Hing WA. Physiological response to water immersion: a method for sport recovery? Sports Med 2006; 36: 747–65.PubMedCrossRefGoogle Scholar
  43. 43.
    Moore KW, O’Garra A, de Waal Malefyt R, Vieira P, Mosmann TR. Interleukin-10. Annu Rev Immunol 1993; 11: 165–90.PubMedCrossRefGoogle Scholar
  44. 44.
    Pretolani M. Interleukin-10: an anti-inflammatory cytokine with therapeutic potential. Clin Exp Allergy 1999; 29: 1164–71.PubMedCrossRefGoogle Scholar
  45. 45.
    Miller E, Markiewicz L, Saluk J, Majsterek I. Effect of short-term cryostimulation on antioxidative status and its clinical applications in humans. Eur J Appl Physiol 2012; 112: 1645–52.PubMedCentralPubMedCrossRefGoogle Scholar
  46. 46.
    Lubkowska A, Dolegowska B, Szygula Z. Whole-body cryostimulation - potential beneficial treatment for improving antioxidant capacity in healthy men- significance of the number of sessions. PLoS One 2012; 7: e46352.CrossRefGoogle Scholar
  47. 47.
    Lubkowska A, Dolegowska B, Szygula Z, Klimek A. Activity of selected enzymes in erythrocytes and level of plasma antioxidants in response to single whole-body cryostimulation in humans. Scand J Clin Lab Invest 2009; 69: 387–94.PubMedCrossRefGoogle Scholar
  48. 48.
    Chen JL, Wu Y. Cardiovascular risk factors in Chinese American children: associations between overweight, acculturation, and physical activity. J Pediatr Health Care 2008; 22: 103–10.PubMedCentralPubMedCrossRefGoogle Scholar
  49. 49.
    Drygas W, Kostka T, Jegier A, Kunski H. Long-term effects of different physical activity levels on coronary heart disease risk factors in middle-aged men. Int J Sports Med 2000; 21: 235–41.PubMedCrossRefGoogle Scholar
  50. 50.
    McMurray RG, Bangdiwala SI, Harrell JS, Amorim LD. Adolescents with metabolic syndrome have a history of low aerobic fitness and physical activity levels. Dyn Med 2008; 7: 5.PubMedCentralPubMedCrossRefGoogle Scholar
  51. 51.
    Fernandez ML, Webb D. The LDL to HDL cholesterol ratio as a valuable tool to evaluate coronary heart disease risk. J Am Coll Nutr 2008; 27: 1–5.PubMedCrossRefGoogle Scholar
  52. 52.
    Tangvarasittichai S, Poonsub P, Tangvarasittichai O. Association of serum lipoprotein ratios with insulin resistance in type 2 diabetes mellitus. Indian J Med Res 2010; 131: 641–8.PubMedGoogle Scholar
  53. 53.
    Xiang SK, Hua F, Tang Y, et al. Relationship between Serum Lipoprotein Ratios and Insulin Resistance in Polycystic Ovary Syndrome. Int J Endocrinol 2012; 2012: 173281.PubMedCentralPubMedCrossRefGoogle Scholar
  54. 54.
    Pedersen BK, Febbraio MA. Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiol Rev 2008; 88: 1379–406.PubMedCrossRefGoogle Scholar

Copyright information

© John Libbey Eurotext 2014

Authors and Affiliations

  • Ewa Ziemann
    • 1
    • 5
    Email author
  • Robert A. Olek
    • 2
  • Tomasz Grzywacz
    • 1
  • Jan J. Kaczor
    • 3
  • Jędrzej Antosiewicz
    • 4
  • Wojciech Skrobot
    • 3
  • Sylwester Kujach
    • 1
  • Radosław Laskowski
    • 1
  1. 1.Department of PhysiologyGdansk University of Physical Education and SportGdańskPoland
  2. 2.Department of BiochemistryGdansk, University of Physical Education and SportGdańskPoland
  3. 3.Department of PhysiotherapyGdansk, University of Physical Education and SportGdańskPoland
  4. 4.Department of Bioenergetics and Physiology of ExerciseMedical University of GdanskGdańskPoland
  5. 5.Department of PhysiologyGdansk University of Physical Education and SportGdanskPoland

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