, Volume 28, Issue 2, pp 311–323

Testate amoebae as palaeohydrological proxies in sürmene ağaçbaşi yaylasi peatland (northeast Turkey)

  • Richard J. Payne
  • Dan J. Charman
  • Sean Matthews
  • Warren J. Eastwood


Testate amoebae are unicellular micro-organisms whose hydrological sensitivity and good preservation in peats make them valuable proxies for past peatland surface wetness, and therefore climate. Previous testate amoebae transfer functions have been spatially restricted with no studies from Asia. To derive a transfer function, a sequence of samples was extracted from an ombrotrophic peatland in Turkey and amoebae counted. The internal structure of the data was explored using principal components analysis and relationships with the environmental data tested by redundancy analyses. Transfer function models were developed using a variety of techniques. As in other regions, depth to water table is the most important control on amoebae community composition. Transfer function performance was initially poor, primarily due to the inclusion of samples from areas of the site that had been heavily affected by peat cutting and had distinctly different amoebae communities. Model performance is improved by selective sample exclusion, reducing jack-knifed root mean square error of prediction to 7.1 cm. The model was tested using an initial palaeoecological data-set. Overlap with the training set was limited, although a hydrological reconstruction using this model produces similar results to a transfer function derived from northern European peatlands. This study provides the first testate amoebae transfer function from Asia and demonstrates that hydrological preferences of many of the key taxa are consistent across a large area of the Northern Hemisphere. The transfer function will allow detailed palaeoclimate reconstruction from this peatland, adding to our knowledge of Holocene climatic change in southwest Asia.

Key Words

Asia Minor paleoclimate protists transfer function 


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Literature Cited

  1. Baker, A., C. J. Caseldine, M. A. Gilmour, D. Charman, C. J. Proctor, C. J. Hawkesworth, and N. Phillips. 1999. Stalagmite luminescence and peat humification records of palaeomoisture for the last 2500 years. Earth and Planetary Science Letters 165: 157–62.CrossRefGoogle Scholar
  2. Bar-Matthews, M., A. Ayalon, and A. Kaufman. 1998. Middle to Late Holocene (6,500 yr. period) paleoclimate in the Eastern Mediterranean region from stable isotopic composition of speleothems from Soreq Cave, Israel. In A. S. Issar and N. Brown (eds.) Water, Environment and Society in Times of Climatic Change. Kluwer, Dordrecht, The Netherlands.Google Scholar
  3. Birks, H. J. B. 1995. Quantitative palaeoecological reconstructions. In D. Maddy and S. Brew (eds.) Statistical Modelling of Quaternary Science Data. Quaternary Research Association, Cambridge, UK.Google Scholar
  4. Blackford, J. 2000. Palaeoclimatic records from peat bogs. Trends in Ecology and Evolution 15: 193–98.CrossRefPubMedGoogle Scholar
  5. Bobrov, A., D. Charman, and B. Warner. 1999. Ecology of testate amoebae (Protozoa: Rhizopoda) on peatlands in Western Russia with special attention to niche separation in closely related taxa. Protist 150: 125–36.CrossRefPubMedGoogle Scholar
  6. Booth, R. K. 2001. Ecology of testate amoebae (protozoa) in two Lake Superior coastal wetlands: implications for palaeoecology and environmental monitoring. Wetlands 21: 564–76.CrossRefGoogle Scholar
  7. Booth, R. K. 2002. Testate amoebae as paleoindicators of surface-moisture changes on Michigan peatlands: modern ecology and hydrological calibration. Journal of Paleolimnology 28: 329–48.CrossRefGoogle Scholar
  8. Booth, R. K. and J. R. Zygmunt. 2005. Biogeography and comparative ecology of testate amoebae inhabiting Sphagnum-dominated peatlands in the Great Lakes and Rocky Mountain regions of North America. Diversity and Distributions 11: 577–90.CrossRefGoogle Scholar
  9. Byfield, A. and N. Őzhatay. 1997. A future for Turkey’s peatlands: a conservation strategy for Turkey’s peatland heritage. Doğal Hayati Koruma Derneği, Istanbul, Turkey.Google Scholar
  10. Çayci, G., Y. Ataman, I. Űnver, and N. Munsuz. 1988. Distribution and horticultural values of the peats in Anatolia. Acta Horticulturae 238: 189–96.Google Scholar
  11. Charman, D. 1997. Modelling hydrological relationships of testate amoebae (Protozoa: Rhizopoda) on New Zealand peatlands. Journal of the Royal Society of New Zealand 27: 465–83.Google Scholar
  12. Charman, D. J. 2001. Biostratigraphic and palaeoenvironmental applications of testate amoebae. Quaternary Science Reviews 20: 1753–64.CrossRefGoogle Scholar
  13. Charman, D. J., A. Blundell, ACCROTELM members. 2007. A pan-European testate amoebae transfer function for palaeohydrological reconstruction on ombrotrophic peatlands. Journal of Quaternary Science.Google Scholar
  14. Charman, D. J., A. Blundell, R. C. Chiverrell, D. Hendon, and P. G. Langdon. 2006. Compilation of non-annually resolved Holocene proxy-climate records: stacked Holocene peatland palaeo-water table reconstructions from northern Britain. Quaternary Science Reviews 25: 336–50.CrossRefGoogle Scholar
  15. Charman, D. J., A. D. Brown, D. Hendon, A. Kimmel, and E. Karofeld. 2004. Testing the relationship between Holocene peatland palaeoclimate reconstructions and instrumental data. Quaternary Science Reviews 23: 137–43.CrossRefGoogle Scholar
  16. Charman, D. and D. Hendon. 2000. Long-term changes in soil water tables over the past 4500 years: relationships with climate and North Atlantic atmospheric circulation and sea surface temperatures. Climatic Change 47: 45–59.CrossRefGoogle Scholar
  17. Charman, D., D. Hendon, and W. Woodland. 2000. The Identification of testate amoebae (protozoa: rhizopoda) from British oligotrophic peats. Quaternary Research Association Technical Guide Series, Cambridge, UK.Google Scholar
  18. Charman, D. and B. Warner. 1992. Relationship between testate amoebae (Protozoa: Rhizopoda) and microenvironmental parameters on a forested peatland in northeastern Ontario. Canadian Journal of Zoology 7: 2474–82.CrossRefGoogle Scholar
  19. Charman, D. and B. Warner. 1997. The ecology of testate amoebae (Protozoa: Rhizopoda) and microenvironmental parameters in Newfoundland, Canada: modeling hydrological relationships for palaeoenvironmental reconstruction. Ecoscience 4: 555–62.Google Scholar
  20. Clarke, K. J. 2003. Guide to the Identification of Soil Protozoa — Testate Amoebae Special publication 12. Freshwater Biological Association, Ambleside, UK.Google Scholar
  21. Corbet, S. A. 1973. An illustrated introduction to the Testate Rhizopods in Sphagnum, with special reference to the area around Malham Tarn, Yorkshire. Field Studies 3: 801–38.Google Scholar
  22. Ellison, R. L. and C. G. Ogden. 1987. A guide to the study and identification of fossil testate amoebae in Quaternary lake sediments. International Review of Hydrobiology 72: 639–52.CrossRefGoogle Scholar
  23. Goodfriend, G. A. 1999. Terrestrial stable isotope records of Late Quaternary paleoclimates in the eastern Mediterranean region. Quaternary Science Reviews 18: 501–14.CrossRefGoogle Scholar
  24. Hendon, D. and D. Charman. 1997. The preparation of testate amoebae (Protozoa: Rhizopoda) samples from peat. The Holocene 7: 199–205.CrossRefGoogle Scholar
  25. Hendon, D., D. Charman, and M. Kent. 2001. Palaeohydrological records from testate amoebae analysis from peatlands in northern England: within-site variability, between-site variability, between-site comparability and palaeoclimatic implications. The Holocene 11: 127–48.CrossRefGoogle Scholar
  26. Jones, M. D., M. J. Leng, C. N. Roberts, M. Türkeş, and R. Moyeed. 2005. A coupled calibration and modelling approach to the understanding of dry-land lake oxygen isotope records. Journal of Paleolimnology 34: 391–411.CrossRefGoogle Scholar
  27. Jones, M. D., C. N. Roberts, M. J. Leng, and M. Türkeş. 2006. A high-resolution late Holocene lake isotope record from Turkey and links to North Atlantic and monsoon climate. Geology 34: 361–64.CrossRefGoogle Scholar
  28. Juggins, S. 2003. C2 user guide. Software for ecological and palaeoecological data analysis and visualisation. University of Newcastle, Newcastle upon Tyne, UK.Google Scholar
  29. Kashima, K. 2002. Environmental and climatic changes during the last 20,000 years at Lake Tuz, central Turkey. Catena 48: 3–20.CrossRefGoogle Scholar
  30. Lamentowicz, M. and E. A. D. Mitchell. 2005. The ecology of testate amoebae (Protists) in Sphagnum in relation to peatland ecology. Microbial Ecology 50: 48–63.CrossRefPubMedGoogle Scholar
  31. Mauquoy, D., M. Blaauw, B. van Geel, A. Borromei, M. Quattrocchio, F. M. Chambers, and G. Possnert. 2004. Late Holocene climatic changes in Tierra del Fuego based on multiproxy analyses of peat deposits. Quaternary Research 61: 148–58.CrossRefGoogle Scholar
  32. Mitchell, E., B. Warner, A. Buttler, and J.-M. Gobat. 1999. Ecological patterns of testate amoebae (Protozoa) on peatlands in the Jura Mountains, Switzerland and France. Ecoscience 6: 565–76.Google Scholar
  33. Ogden, C. G. 1983. Observations on the systematics of the genus Difflugia in Britain (Rhizopoda, Protozoa). Bulletin of the British Museum of Natural History (Zoology) 44: 1–73.Google Scholar
  34. Ogden, C. G. and R. H. Hedley. 1980. An atlas of freshwater testate amoebae. British Museum (Natural History) and Oxford University Press, London and Oxford, UK.Google Scholar
  35. Őz, D. 1996. Peatlands in Turkey. In E. Lappalainen (ed.) Global Peat Resources. International Peat Society, Jyskä, Finland.Google Scholar
  36. Page, E. B. 1963. Ordered hypotheses for multiple treatments: A significance test for multiple ranks. Journal of the American Statistical Association 58: 216–30.CrossRefGoogle Scholar
  37. Payne, R., W. Eastwood, and D. Charman. 2007. The ongoing destruction of Turkey’s largest upland mire by peat cutting. International Mire Conservation Group Newsletter 2007/1.Google Scholar
  38. Payne, R., K. Kishaba, J. Blackford, and E. Mitchell. 2006. The ecology of testate amoebae in southcentral Alaskan peatlands: Building transfer function models for palaeoenvironmental inference. The Holocene 16: 403–14.CrossRefGoogle Scholar
  39. Payne, R. and E. Mitchell. 2007. Ecology of testate amoebae from mires in the Central Rhodope Mountains, Greece and development of a transfer function for paleohydrological reconstruction. Protist 158: 159–71.CrossRefPubMedGoogle Scholar
  40. Payne, R., W. Eastwood, and D. Charman. 2007. The ongoing destruction of Turkey’s largest upland mire by peat cutting. International Mire Conservation Group Newsletter 2007/1.Google Scholar
  41. Roberts, N., J. M. Reed, M. J. Leng, C. Kuzucuoğlu, M. Fontugne, J. Bertaux, H. Woldring, S. Bottema, S. Black, E. Hunt, and M. Karabiyikoglu. 2001. The tempo of Holocene climatic change in the eastern Mediterranean region: new high-resolution crater-lake sediment data from central Turkey. The Holocene 11: 721–36.CrossRefGoogle Scholar
  42. Schoning, K., D. J. Charman, and S. Wastegård. 2005. Reconstructed water tables from two ombrotrophic mires in eastern central Sweden compared with instrumental meteorological data. The Holocene 15: 111–18.CrossRefGoogle Scholar
  43. Schilman, B., M. Bar-Matthews, A. Almogi-Labin, and B. Luz. 2001. Global climate instability reflected by Eastern Mediterranean marine records during the late Holocene. Palaeogeography, Palaeoclimatology, Palaeoecology 176: 157–76.CrossRefGoogle Scholar
  44. Stevens, L. R., H. E. Wright Jr., and E. Ito. 2001. Proposed changes in seasonality of climate during the late-glacial and Holocene at Lake Zeribar, Iran. The Holocene 11: 747–56.CrossRefGoogle Scholar
  45. Ter Braak, C. and P. Šmilauer. 1997–2004. CANOCO for Windows. Biometris-Plant Research, The Netherlands.Google Scholar
  46. Tolonen, K., B. Warner, and H. Vasander. 1994. Ecology of testaceans (protozoa:rhizopoda) in mires in Southern Finland: II multivariate analysis. Archiv fur Protistenkunde 144: 97–112.Google Scholar
  47. Türkeş, M. and U. M. Sümer. 2004. Spatial and temporal patterns of trends and variability in diurnal temperature ranges of Turkey. Theoretical and Applied Climatology 77: 195–227.CrossRefGoogle Scholar
  48. Türkeş, M., U. M. Sümer, and G. Kiliç. 2007. Variations and trends in annual mean air temperatures in Turkey with respect to climatic variability. International Journal of Climatology 15: 557–69.CrossRefGoogle Scholar
  49. Warner, B. and D. Charman. 1994. Holocene changes on a peatland interpreted from testate amoebae (Protozoa) analysis. Boreas 23: 270–80.CrossRefGoogle Scholar
  50. Wick, L., G. Lemcke, and M. Sturm. 2003. Evidence of Lateglacial and Holocene climatic change and human impact in eastern Anatolia: high-resolution pollen, charcoal, isotopic and geochemical records from the laminated sediments of Lake Van, Turkey. The Holocene 13: 665–75.CrossRefGoogle Scholar
  51. Wilkinson, D. 2001. What is the upper size limit for cosmopolitan distribution in free-living microorganisms? Journal of Biogeography 28: 285–91.CrossRefGoogle Scholar
  52. Wilmshurst, J., S. Wiser, and D. Charman. 2003. Reconstructing Holocene water tables in New Zealand using testate amoebae: differential preservation of tests and implications for the use of transfer functions. The Holocene 13: 61–72.CrossRefGoogle Scholar
  53. Woodland, W., D. Charman, and P. Simms. 1998. Quantitative estimates of water tables and soil moisture in Holocene peatlands from testate amoebae. The Holocene 8: 261–73.CrossRefGoogle Scholar

Copyright information

© The Society of Wetland Scientists 2008

Authors and Affiliations

  • Richard J. Payne
    • 1
  • Dan J. Charman
    • 2
  • Sean Matthews
    • 2
  • Warren J. Eastwood
    • 3
  1. 1.The Fitch LaboratoryBritish School at AthensAthinaGreece
  2. 2.School of GeographyUniversity of PlymouthPlymouthUK
  3. 3.School of Geography, Earth and Environmental SciencesThe University of BirminghamEdgbastonUK
  4. 4.Geography, School of Environment and DevelopmentThe University of ManchesterManchesterUK

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