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, Volume 57, Issue 4, pp 367–381 | Cite as

Molecular systematics of the Old World Astragalus (Fabaceae) as inferred from nrDNA ITS sequence data

  • S. Kazempour Osaloo
  • A. A. Maassoumi
  • N. Murakami
Systematics

Abstract

This study represents a nuclear rDNA ITS-based phylogenetic analyses of a greater sampling of the Old WorldAstragalus compared to our previous work (212 vs. 134 taxa). Phylogenetic relationships among 212 species (213 accessions) of the Old WorldAstragalus, including newly segregated monotypic genusPodlechiella, the two aneuploid New WorldAstragalus, and five related genera, were inferred from analyses of nuclear rDNA ITS sequences using maximum parsimony. A total of 658 nucleotide sites and four binary characters for indels were analyzed. The results of phylogenetic analyses suggest sect.Phyllolobium, comprising mostly the Chinese species, is placed outside of the so-calledAstragalus s. str. and is a well-supported monophyletic group. The monotypic annual segregate genusThlaspidium (≡Astragalus sect.Thlaspidium, A. thlaspi), is clearly nested withinAstragalus s. str. Among the many sections analyzed here, only sects.Cenanthrum, Caraganella, Eremophysa, Incani, Laxiflori, andLotidium are strongly supported as monophyletic. Our analysis, in agreement with previous studies, shows that the North American euploidAstragalus species are scattered throughout the Old World groups of the genus.

Key words

Astragalus Fabaceae nrDNA ITS Phyllolobium phylogeny Podlechiella Thlaspidium 

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Literature Cited

  1. Barneby, R. 1964. Atlas of North AmericanAstragalus. Memoirs of The New York Botanical Garden 13: 1–1188.Google Scholar
  2. Bunge, A. 1868. GenerisAstragali species Gerontogeae. Pars prior. Claves diagnosticae. Mémoires de l’Académie Impériale des Sciences de St. Pétersbourg 11(16): 1–140.Google Scholar
  3. — 1869. GenerisAstragali species Gerontogeae. Pars altera. Specierum enumeratio. diagnosticae. Mémoires de l’Académie Impériale des Sciences de St. Pétersbourg 15(1): 1–245.Google Scholar
  4. Catalán, P., E. A. Kellogg &R. G. Olmstead. 1997. Phylogeny of Poaceae subfamily Pooideae base on by chloroplastndhF gene DNA sequences. Molecular Phylogenetics and Evolution 8: 150–166.PubMedCrossRefGoogle Scholar
  5. Deml, I. 1972. Revision der SektionenAcanthophace Bunge undAegacantha Bunge der GattungAstragalus L. Boissiera 21: 1–235.Google Scholar
  6. Dong, T. T. X., X. Q. Ma, C. Clarke, Z. H. Song, Z. N. Ji, C. K. Lo &K. W. K. Tsim. 2003. Phylogeny ofAstragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS and 18S rRNA. Journal of Agriculture and Food Chemistry 51: 6709–6714.CrossRefGoogle Scholar
  7. Downie, S. R., S. Ramanath, D. S. Katz-Downie &E. Llanas. 1998. Molecular systematics of Apiaceae subfamily Apioideae: phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacer and plastidrpoC1 intron sequences. American Journal of Botany 85: 563–591.CrossRefGoogle Scholar
  8. Doyle, J. J. &J. L. Doyle. 1987. A rapid DNA Isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Google Scholar
  9. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.CrossRefGoogle Scholar
  10. Fischer, F. B. 1818.Phyllolobium chinense. Page 33.In: C. Sprengel, editor. Novi Proventus. Halae.Google Scholar
  11. Fu, K. T. 1993.Astragalus. Pages 78–349.In: K. T. Fu, editor. Flora Reipublicae Popularis Sinicae. Vol. 42 (1). Science Press, Beijing (In Chinese).Google Scholar
  12. Ghahremani-nejad, F. 2004. The sections ofAstragalus L. with bifurcating hairs in Iran. Turkish Journal of Botany 28: 101–117.Google Scholar
  13. Hutchinson, J. 1964. The genera of flowering plants. Vol. I. Clarendon Press, Oxford.Google Scholar
  14. Ikonnikov, S. 1977. Notae de flora Badachschanica, 4. Novosti Sistematiki Vysshikh Rastenii 14: 231–232.Google Scholar
  15. Kang, Y., M.-L. Zhang &Z.-D. Chen. 2003. A preliminary phylogenetic study of the subgenusPogonophace (Astragalus) in China based on ITS sequence data. Acta Botanica Sinica 45: 140–145.Google Scholar
  16. —. 2004. Study of pollen brush in selected species ofAstragalus L. subgenusPogonophace Bunge (Leguminosae). Plant Systematics and Evolution 249: 1–8.CrossRefGoogle Scholar
  17. Kazempour Osaloo, S., A. A. Maassoumi &N. Murakami. 2003. Molecular systematics of the genusAstragalus L. (Fabaceae): phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast genendhF sequences. Plant Systematics and Evolution 242: 1–32.CrossRefGoogle Scholar
  18. Lavin, M. &S. A. Delgado. 1990. Pollen brush of Papilionoideae (Leguminosae): morphological variation and systematic utility. American Journal of Botany 77: 1294–1312.CrossRefGoogle Scholar
  19. Liston, A. &J. A. Wheeler. 1994. The phylogenetic position of the genusAstragalus (Fabaceae): evidence from the chloroplast genesrpoC1 andrpoC2. Biochemical Systematics and Ecology 22: 377–388.CrossRefGoogle Scholar
  20. Lock, J. M. & K. Simpson. 1991. Legumes of West Asia, a check list. Royal Botanical Gardens, Kew.Google Scholar
  21. Maassoumi, A. A. 1989. The genusAstragalus in Iran. Vol. 2. Perennials. Research Institute of Forests and Rangeland, Tehran.Google Scholar
  22. — 1993. Revision ofAstragalus L. sect. Malacothrix Bunge (Leguminosae) in Iran. Sendtnera 1: 157–240.Google Scholar
  23. — 1998.Astragalus in the Old World, Check-List. Research Institute of Forests and Rangeland, Tehran.Google Scholar
  24. — 2000. The genusAstragalus in Iran, Vol. 4. Perennials. Research Institute of Forests and Rangeland, Tehran.Google Scholar
  25. — 2003. Papilionaceae (Astragalus I). Pages 1–386.In: M. Assadi & A. A. Maassoumi editors. Flora of Iran, No. 43. Research Institute of Forests and Rangeland, Tehran.Google Scholar
  26. —,A. Ghahreman, F. Ghahreman-mejad &F. Matin. 1999.Astragalus gigantirostratus (Fabaceae), a remarkable new species from N. Iran and supplementary notes onA. sectionCytisodes Bunge. Willdenowia 29: 21–225.Google Scholar
  27. Podlech, D. 1975. Revision der SektionCaraganella Bunge der GattungAstragalus L. Mitteilungen (aus) der Botanischen Staatssammlung München 12: 153–166.Google Scholar
  28. — 1982. Neue Aspekte zur Evolution und Gliederung der GattungAstragalus L. Mitteilungen (aus) der Botanischen Staatssammlung München 18: 359–378.Google Scholar
  29. — 1983. Zur Taxonomie und Nomenclatur der TragacanthoidenAstragali. Mitteilungen (aus) der Botanischen Staatssammlung München 19: 1–23.Google Scholar
  30. — 1986. Taxonomic and phytogeographical problems inAstragalus of the Old World and South-Wrest Asia. Proceedings of the Royal Society of Edinburgh 89B: 37–43.Google Scholar
  31. — 1991. The systematics of the annual species of the genusAstragalus L. (Leguminosae). Flora et Vegetatio Mundi 9: 1–8.Google Scholar
  32. — 1994. Revision der altweltlichen anuellen Arten der GattungAstragalus L. (Leguminosae). Sendtnera 2: 39–170.Google Scholar
  33. — 1998. Phylogeny and progression of characters in Old WorldAstragali (Leguminosae). Pages 405–407.In: A. Zhang & S. Wu, editors. Floristic characteristics and diversity of east Asian plants. China Higher Education Press, Beijing.Google Scholar
  34. — 1999. Papilionaceae III,Astragalus I. Pages 1–350.In: K. H. Rechinger, editor. Flora Iranica, No. 174. Akademische Druck-u. Verlagsanstalt, Graz.Google Scholar
  35. —,Sh. Zarre &A. A. Maassoumi. 2001. Papilionaceae IV.Astragalus II. Pages 1–197.In: K. H. Rechinger, editor Flora Iranica, No. 175. Avesa Akademische Verlagsgesellschaft, Salzburg.Google Scholar
  36. Polhill, R. M. 1981. Galegeae. Pages 357–363.In: R. M. Polhill & P. H. Raven, editors. Advances in Legume systematics. Part 1. Royal Botanical Gardens, Kew.Google Scholar
  37. Rechinger, K. H. 1958. Leguminosae.In: Koeie & K. H. Rechinger, Symbolae Afghanicae III. Biologiske Skrifter 9(3): 1–208.Google Scholar
  38. Sanderson, M. J. &A. Liston. 1995. Molecular phylogenetic systematics of Galegeae, with special reference toAstragalus. Pages 331–350.In: M. Crisp & J. J. Doyle, editors. Advances in Legume systematics 7: Phylogeny. Royal Botanical Gardens, Kew.Google Scholar
  39. —. 1996. Diversification rates in a temperate legume clade: are there so many species ofAstragalus (Fabaceae). American Journal of Botany 83: 1488–1502.CrossRefGoogle Scholar
  40. Swofford, D. L. 2002. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.Google Scholar
  41. Wenninger, J. 1992. Revision vonAstragalus L. sect.Chlorostachys Bunge sect.Phyllolobium (Fisch.) Bunge und sect.Skythropos Simpson (Leguminosae). Mitteilungen (aus) der Botanischen Staatssammlung München 30: 1–196.Google Scholar
  42. Wojciechowski, M. F., M. J. Sanderson, B. G. Baldwin &M. J. Donoghue. 1993. Monophyly of aneuploidAstragalus (Fabaceae): evidence from nuclear ribosomal DNA internal transcribed spacer sequences. American Journal of Botany 80: 711–722.CrossRefGoogle Scholar
  43. —. 1999. Evidence on the monophyly ofAstragalus (Fabaceae) and its major subgroups based on nuclear ribosomal DNA ITS and chloroplast DNAtrnL intron data. Systematic Botany 24: 409–437.CrossRefGoogle Scholar
  44. Zarre, Sh. 2003. Hair micromorphology and its phylogenetic application in thorny species of Astragalus (Fabaceae). Botanical Journal of the Linnean Society 143: 323–330.CrossRefGoogle Scholar
  45. —. 2001.Astragalus sect.Semnanenses (Fabaceae): a new monotypic section from Iran. Nordic Journal of Botany 21: 485–491.CrossRefGoogle Scholar

Copyright information

© The New York Botanical Garden 2005

Authors and Affiliations

  • S. Kazempour Osaloo
    • 1
  • A. A. Maassoumi
    • 2
  • N. Murakami
    • 3
  1. 1.Department of Plant BiologyTarbiat Modares UniversityTehranIran
  2. 2.Department of BotanyResearch Institute of Forests and RangelandsTehranIran
  3. 3.Department of Botany, Graduate School of ScienceKyoto UniversityKyotoJapan

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