概要
现有的可逆性梗阻性黄疸动物模型存在许多缺陷,严重限制了其应用。一个简单、可靠的可逆性梗阻性黄疸动物模型值得进一步研究和探索。本研究利用改良式胆管结扎代替传统胆管结扎构建SD大鼠梗阻性黄疸模型,并基于改良式胆管结扎成功构建可逆性梗阻性黄疸模型。并通过肝功能、苏木精-伊红(HE)染色、Masson染色及天狼星红染色研究证实了胆道复通的有效性。进一步研究发现胆道复通7天后胆管细胞增殖和肝纤维化明显减轻,但其组织学病变尚未完全恢复正常;同时胆道复通7天后可显著降低CK19和衰老相关蛋白(p16及p21)的表达,但较正常组织仍高表达。因此,本研究成功建立了一种简单有效的可逆性梗阻性黄疸模型,其可有效地避免二次剖腹手术,显著降低死亡率,具有广泛的应用前景。
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Nos. 51803153 and 81970548), the Wuhan Science and Technology Project (No. 2019020701011485), the Medical Science Advancement Program (Clinical Medicine) of Wuhan University (No. TFLC 2018003), and the Zhongnan Hospital of Wuhan University Science, Technology, and Innovation Seed Fund (No. CXPY2020049), China.
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Yongkang ZOU designed the research, established the animal models, analyzed the date, and wrote the original the manuscript. Pengpeng YUE performed the experiment and analyzed the experimental data. Hankun CAO and Liqin WU analyzed the experimental data. Li XU was responsible for collecting the relevant literature. Zhongzhong LIU guided the experiment. Shuangquan WU guided the experiment and revised this manuscript. Qifa YE contributed to funding acquisition and project administration, designed the study, and revised this manuscript. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Detailed methods are provided in the electronic supplementary materials of this paper.
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Yongkang ZOU, Pengpeng YUE, Hankun CAO, Liqin WU, Li XU, Zhongzhong LIU, Shuangquan WU, and Qifa YE declare that they have no conlfict of interest.
All animal procedures were performed in accordance with the Experimental Animal Management Ordinance of Wuhan University. This research was approved by the Institutional Animal Care and Use Committee of Wuhan University Center for Animal Experiments (No. WP20210013).
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Fig. S1; Materials and methods
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Zou, Y., Yue, P., Cao, H. et al. A novel ameliorated rat model of reversible obstructive jaundice. J. Zhejiang Univ. Sci. B 24, 345–351 (2023). https://doi.org/10.1631/jzus.B2200421
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DOI: https://doi.org/10.1631/jzus.B2200421