概要
为提高临床肿瘤检测效率和准确性,我们通过制备负载碳量子点(CQDs)的纳米泡进行超声荧光双重检测,探索多重成像。在本实验中,我们采用薄膜分散法制备了阳离子脂质体,并使用全氟戊烷作为纳米气泡的核心气体材料。通过电荷效应将纳米气泡与带负电荷的碳量子点耦合,制备出利用超声对比和荧光检测进行双向诊断的检测剂,并对负载碳量子点脂质体纳米气泡的配方和制备进行了筛选。当全氟戊烷用量为5 µL,胆固醇与(2,3-二油酰基-丙基)-三甲基氯化铵(DOTAP)的质量比为1:1,在超声功率为20%,时间为1 s的条件下,纳米泡的平均粒径为(224.6±2.4)nm,平均zeta电位为(46.2±1.3)mV。体内实验表明,纳米气泡可以在5分钟内富集到肿瘤部位,使超声成像更加清晰,有利于肿瘤的检测。载有CQDs的脂质体纳米泡有望成为一种新的临床应用的超声造影剂,可以为肿瘤诊断提供依据,并为肿瘤治疗提供机会。
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 21873057), the Shandong Provincial Natural Science Foundation of China (No. ZR2019MB041), and the Fundamental Research Funds of Shandong University (No. 2018JC006), China.
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Yankun ZHANG performed the experimental research and data analysis, wrote and edited the manuscript. Yu XIN and Qiong WU performed the establishment of animal models. Lan LIU and Yunxia WANG designed the experiments and wrote this article. Bingtao TANG and Kongxi ZHU gave some good suggestions on the revision of the manuscript, polished English, and checked the final version. Guimei LIN and Hongjuan WANG guided the experiment. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Yankun ZHANG, Bingtao TANG, Yu XIN, Qiong WU, Lan LIU, Yunxia WANG, Kongxi ZHU, Guimei LIN, and Hongjuan WANG declare that they have no conflict of interest. All institutional and national guidelines for the care and use of laboratory animals were followed.
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Materials and methods; Table S1; Fig. S1
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Zhang, Y., Tang, B., Xin, Y. et al. Nanobubbles loaded with carbon quantum dots for ultrasonic fluorescence dual detection. J. Zhejiang Univ. Sci. B 23, 778–783 (2022). https://doi.org/10.1631/jzus.B2200233
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DOI: https://doi.org/10.1631/jzus.B2200233