概述
目的
脆江蓠是中国沿海广泛分布的重要经济红藻, 多糖是其主要生物成分, 通过对其多糖分离纯化, 化学表征及活性评价, 为脆江蓠的开发奠定实验基础。
创新点
首次从脆江蓠中得到均一硫酸化多糖GCP-3A, 并发现其多糖对巨噬细胞RAW264.7具有显著激活作用。
方法
以脆江蓠为原料, 采用水提醇沉, 柱层析等方法分离纯化得到脆江蓠硫酸化多糖GCP-3A; 采用凝胶渗透色谱(GPC), 气相色谱-质谱(GC-MS), 傅里叶变换红外光谱(FT-IR)和核磁共振(NMR)探究其化学特征; 采用扫描电镜(SEM)和刚果红研究其表面形态; 通过噻唑蓝(MTT)和一氧化氮(NO)试验分析其对RAW264.7巨噬细胞的活化作用。
结论
GCP-3A是一种硫酸化酸性多糖, 分子量为11.87 kDa, 由木糖, 半乳糖, 葡萄糖和甘露糖组成, 四者的摩尔比为3.00:29.28:0.63:0.45, 且含有α,β-糖苷键。GCP-3A是一种由不规则片状和棒状交织而成的异质多糖, 无三螺旋构象。此外, GCP-3A显著性地促进了RAW264.7巨噬细胞的增殖和NO分泌, 具有应用于医药及功能性食品的潜力。
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 81572989) and the International Academic Cooperation of Science and Technology Committee of Shanghai, China (No. 18430721100).
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Feifei LI performed the experimental research and data analysis, and wrote and edited the manuscript. Kehai LIU contributed to the study design, data analysis, and editing of the manuscript. Kewu LIU contributed to the study design and editing of the manuscript. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Feifei LI, Kehai LIU, and Kewu LIU declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Li, F., Liu, K. & Liu, K. Chemical characterization of a new sulfated polysaccharide from Gracilaria chouae and its activation effects on RAW264.7 macrophages. J. Zhejiang Univ. Sci. B 23, 84–88 (2022). https://doi.org/10.1631/jzus.B2100508
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DOI: https://doi.org/10.1631/jzus.B2100508