概要
肝细胞癌 (HCC) 具有复杂的肿瘤内异质性, 这会导致治疗失败. 传统医学和实验研究表明, 食用昆虫具有治疗包括 HCC 在内的不同疾病的潜力. 我们在获得黄粉虫水提物后, 通过组织病理学、 免疫组织化学、 蛋白质印迹和影像学分析确定其有效成分, 并比较黄粉虫三个生命阶段 (包括幼虫、 蛹和成虫) 的水提物对二乙基亚硝胺 (DEN) 诱导的小鼠早期 HCC 的影响. 结果表明, 与黄粉虫蛹的水提取物相比, 幼虫水提物能更显着地通过抗增殖机制来抑制早期肝癌的发生.
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Change history
25 May 2023
An Erratum to this paper has been published: https://doi.org/10.1631/jzus.B21e0201
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Acknowledgments
This work was funded by the National Council of Science and Technology (CONACYT; No. CF2019-53358), the National Institute of Genomic Medicine (INMEGEN; No. 06/2017/I), the Unit of Production and Experimentation of Laboratory Animals of Center for Research and Advanced Studies of the National Polytechnic Institute (UPEAL-CINVESTAV-IPN; No. 0114-14), and the Autonomous University of Hidalgo State (UAEH; No. SEP-PFCE 2018). Brisa Rodope ALARCÓN-SÁNCHEZ gives thanks to CONACYT-Mexico for awarding the masters (No. 484737) and doctoral fellowships (No. 752715). Osiris Germán IDELFONSO-GARCÍA gives thanks to CONACYT-Mexico for awarding the doctoral fellowship (No. 431419). Jaime ARELLANES-ROBLEDO expresses his sincere thanks to the Cátedras-CONACYT program. All authors express their gratitude to DM MORENO-GARCÍA (UAEH), NA LÓPEZ-HERNÁNDEZ, JL CRUZ-COLÍN, and A MUÑOZ-RIVAS (INMEGEN), S HERNÁNDEZ-GARCÍA, E ROMO-MEDINA, L MOLINA-GARCÍA, A CRUZ-HERNÁNDEZ, and C HERNÁNDEZ-CHÁVEZ (CINVESTAV-IPN) for the support in administrative and lab procedures; to J FERNÁNDEZ-HERNÁNDEZ, R LEYVA-MUÑOZ, R GAXIOLA-CENTENO, BE CHÁVEZ-ÁLVAREZ, A ROJO-GARCÍA, MA LÓPEZ-LÓPEZ, I ZAVALA-MEJIA, and ME ZÚÑIGA-ALCÁNTARA (UPEAL-CINVESTAV-IPN) for the assistance with animal breeding, care, and handling; and to NB GABIÑO-LÓPEZ and R NAVA-MONROY (INMEGEN Histology Unit) for the assistance with paraffin-embedding and tissue sectioning.
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Brisa Rodope ALARCÓN-SÁNCHEZ, Osiris Germán IDELFONSO-GARCÍA, Juan OCAMPO-LÓPEZ, Sandra ROSAS-MADRIGAL, and Diana Ivette APARICIO-BAUTISTA performed the experimental research and data analysis. Julio Isael PÉREZ-CARREÓN and Saúl VILLA-TREVIÑO contributed to the study design, writing and editing of the manuscript. Jaime ARELLANES-ROBLEDO and Armando ZEPEDA-BASTIDA contributed to the study conception and design, wrote and edited the manuscript. All authors have read and approved the final manuscript, and therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Armando ZEPEDA-BASTIDA, Juan OCAMPO-LÓPEZ, Brisa Rodope ALARCÓN-SÁNCHEZ, Osiris Germán IDELFONSO-GARCÍA, Sandra ROSAS-MADRIGAL, Diana Ivette APARICIO-BAUTISTA, Julio Isael PÉREZ-CARREÓN, Saúl VILLA-TREVIÑO, and Jaime ARELLANES-ROBLEDO declare that they have no conflict of interest.
All animal experimental procedures were performed in accordance with the Institutional Animal Use and Care Committee of CINVESTAV-IPN and the approved protocol (No. 0114-14).
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Zepeda-Bastida, A., Ocampo-López, J., Alarcón-Sánchez, B.R. et al. Aqueous extracts from Tenebrio molitor larval and pupal stages inhibit early hepatocarcinogenesis in vivo. J. Zhejiang Univ. Sci. B 22, 1045–1052 (2021). https://doi.org/10.1631/jzus.B2100201
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DOI: https://doi.org/10.1631/jzus.B2100201