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Arthrospira maxima (Spirulina) prevents endoplasmic reticulum stress in the kidney through its C-phycocyanin

极大节螺藻(螺旋藻) C-藻蓝蛋白阻碍肾脏内质网胁迫

概要

极大节螺藻 (Arthrospira maxima) 细胞内含有C-藻蓝蛋白, 其具有抗氧化和细胞保护作用, 因此该藻全细胞和C-藻蓝蛋白均被确认为保健品. 然而, 它们的保健效应尚未被充分认识. 由于它们可以降低未折叠蛋白反应, 我们推测, 极大节螺藻细胞及其C-藻蓝蛋白可以防止肾脏内质网胁迫(ERS). 本文旨在研究极大节螺藻细胞及其C-藻蓝蛋白通过ERS反应对氯化汞 (HgCl2) 引起的肾损伤的影响. 实验公鼠先用极大节螺藻细胞或C-藻蓝蛋白处理30 min, 再用5 mg HgCl2/kg体重处理, 之后三天均饲喂藻细胞或C-藻蓝蛋白. 实验鼠安乐死后, 取其肾脏用于分析蛋白激酶RNA样内质网激酶(PERK), 转录激活因子 6(ATF-6) 和需肌醇酶 1α(IRE-1α) 信号途径. 结果表明, 汞中毒会提高PERK/eIF2α/ATF-4/GADD153和IRE-1α/XBP1 途径, 而极大节螺藻细胞处理防止了PERK, ATF-6α和半胱天冬酶的提高;同时, C-藻蓝蛋白处理使得所有这些蛋白得到正常表达. 本研究首次证实了极大节螺藻处理通过其C-藻蓝蛋白的活性防止了HgCl2引发的肾脏ERS.

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Acknowledgments

This study was supported by the Secretaría de Investigación y Posgrado, Instituto Politécnico Nacional (SIP-IPN; Nos. 20200521, 20201091, 20200493, 20200402, and 20200327). We should like to thank Dr. En Tao WANG-HU (Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB)-IPN) for help us with the abstract in Chinese.

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Correspondence to Edgar Cano-Europa.

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Author contributions

Edgar CANO-EUROPA and Margarita FRANCO-COLÍN designed the research. Placido ROJAS-FRANCO and Vanessa BLAS-VALDIVIA conducted the experiments. María Estela MELENDEZ-CAMARGO performed the data analysis. All authors participated in writing the article and have read and approved the final manuscript.

Compliance with ethics guidelines

Placido ROJAS-FRANCO, Margarita FRANCO-COLÍN, Vanessa BLAS-VALDIVIA, María Estela MELENDEZ-CAMARGO, and Edgar CANO-EUROPA declare that they have no conflict of interest.

All experimental procedures described in this research study followed the Mexican Laws and Codes (NOM-062-ZOO-1999). Also, the protocol was approved by the Internal Bioethics Committee of the Escuela Nacional de Ciencias Biológicas (ENCB, IPN), México (CEI-ENCB 019/2014).

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Rojas-Franco, P., Franco-Colín, M., Blas-Valdivia, V. et al. Arthrospira maxima (Spirulina) prevents endoplasmic reticulum stress in the kidney through its C-phycocyanin. J. Zhejiang Univ. Sci. B 22, 603–608 (2021). https://doi.org/10.1631/jzus.B2000725

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关键词

  • C-藻蓝蛋白
  • 极大节螺藻
  • 螺旋藻
  • 肾脏内质网胁迫
  • 肾保护