概要
目的
鉴于风兰(Vanda falcata (Thunb.) Beer)传统繁 殖能力弱,本文拟利用植物组织培养技术建立一 种高效的繁殖体系,以满足市场对风兰数量增多 的需要。
创新点
建立了适用于风兰的高效组培繁殖体系,包括离 体种子萌发、原球茎的形成,以及芽和根的生成。
方法
本研究首先以5 年生母株自花授粉4~5 个月后生 成的种子为试材,依次经70%乙醇和10%次氯酸 钠进行表面消毒后,分别接种于含不同成分的13 种培养基中,统计分析种子萌发情况。进而将形 成原球茎的试材,接种于含不同植物生长调节剂 组合的培养基,统计分析芽和根的生长情况。
结论
评估的13 种不同配方的培养基中,1/2 MS+维生 素+肌醇+20 g/L 蔗糖+3 g/L 活性炭+100 g/L 椰肉 组合可达到100%种子萌发率并形成较大原球 茎;0.2 mg/L α-萘乙酸+0.5 mg/L 6-苄基腺嘌呤组 合具有最高的芽诱导效率;1 mg/L α-萘乙酸+ 0.5 mg/L 6-苄基腺嘌呤+40 g/L蔗糖组合具有最高 的根诱导效率。长势良好的植株经炼苗后可适应 露天栽培。
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References
Anderson AB, 1991. Symbiotic and asymbiotic germination and growth of Spiranthes magnicamporum (Orchidaceae). Lindleyana, 6(4):183–186.
Arditti J, 2008. Micropropagation of Orchids, 2nd Ed. Blackwell Publishing Ltd., Malden, USA, p.778–781.
Arditti J, Michaud JD, Oliva AP, 1981. Seed germination of North American orchids. I. Native California and related species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera. Bot Gaz, 142(4):442–453. https://doi.org/10.1086/337245
Ballard WW, 1990. Further notes on Cypripedium germination. In: Sawyers CE (Ed.), North American Native Terrestrial Orchid Propagation and Production. Chadds Ford Press, Pennsylvania, USA.
Basker S, Narmatha Bai V, 2006. Micropropagation of Coelogyne stricta (D. Don) Schltr. via pseudobulb segment cultures. Trop Subtr Agroecosys, 6:31–35.
Bradburn NM, 1969. The Structure of Psychological Well-being. Aldine Pub. Co., Chicago, USA.
Cardoso JC, Zanello CA, Chen JT, 2020. An overview of orchid protocorm-like bodies: mass propagation, biotechnology, molecular aspects, and breeding. Int J Mol Sci, 21(3):985. https://doi.org/10.3390/ijms21030985
Fast G, 1982. European Terrestrial Orchids (Symbiotic and Asymbiotic Methods). Cornell University Press, New York, USA.
Hossain MM, Sharma M, Teixeira da Silva JA, et al., 2010. Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Sci Hortic, 123(4):479–487. https://doi.org/10.1016/j.scienta.2009.10.009
Kauth PJ, Vendrame WA, Kane ME, 2006. In vitro seed culture and seedling development of Calopogon tuberosus. Plant Cell Tissue Organ Cult, 85(1):91–102. https://doi.org/10.1007/s11240-005-9055-1
Kauth PJ, Dutra D, Johnson TR, et al., 2008. Techniques and applications of in vitro orchid seed germination. In: Teixeira da Silva JA (Ed.), Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. Global Science Books, Ltd., Isleworth, UK, p.375–391.
Kerbauy GB, 1984. Plant regeneration of Oncidium varicosum (Orchidaceae) by means of root tip culture. Plant Cell Rep, 3(1):27–29. https://doi.org/10.1007/BF00270224
Kunakhonnuruk B, Inthima P, Kongbangkerd A, 2018. In vitro propagation of Epipactis flava Seidenf., an endangered rheophytic orchid: a first study on factors affecting asymbiotic seed germination, seedling development and greenhouse acclimatization. Plant Cell Tissue Organ Cult, 135(3):419–432. https://doi.org/10.1007/s11240-018-1475-9
Lawler LJ, 1984. Ethnobotany of the Orchidaceae. In: Arditti J (Ed.), Orchid Biology: Reviews and Perspectives. Cornell University Press, Ithaca.
Mahendran G, Narmatha Bai V, 2009. Mass propagation of Satyrium nepalense D. Don.—a medicinal orchid via seed culture. Sci Hortic, 119(2):203–207. https://doi.org/10.1016/j.scienta.2008.07.029
McKendrick S, 2000. In Vitro Germination of Orchids: A Manual. Ceiba Foundation for Tropical Conservation, p.3-17.
Minea M, Piluek C, Menakanit A, et al., 2004. A study on seed germination and seedling development of Spathoglottis Bl. orchids. Kasetsart J Nat Sci, 38(2):141–156.
Mitchell RB, 1989. Growing hardy orchids from seeds at Kew. Plantsman, 11(3):152–169.
Mitsukuri K, Johkan M, Yamasaki S, et al., 2009a. Effects of anti-browning conditioning on adventitious bud formation in Neofinetia falcata Hu. in vitro. Hortic Environ Biotechnol, 50(4):295–299.
Mitsukuri K, Mori G, Johkan M, et al., 2009b. Effects of explant source and dark-preconditioning on adventitious bud formation in Neofinetia falcata H. H. Hu in vitro. J Jpn Soc Hortic Sci, 78(2):252–256. https://doi.org/10.2503/jjshs1.78.252
Mitsukuri K, Johkan M, Yamasaki S, et al., 2010. L-2-aminooxy-3-phenylpropionic acid (AOPP) controls browning and promotes adventitious bud formation in Neofinetia falcata in vitro. J Jpn Soc Hortic Sci, 79(4):367–371. https://doi.org/10.2503/jjshs1.79.367
Murashige T, Skoog F, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant, 15(3):473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nongdam P, Chongtham N, Tewari R, 2006. In vitro multiplication of Cymbidium pendulum orchids via embryo culture. Plant Cell Biotechnol Mol Biol, 7(3):145–150.
Ohwi J, 1965. Flora of Japan. Smithsonian Institution, Washington, USA.
Palama TL, Menard P, Fock I, et al., 2010. Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biol, 10:82. https://doi.org/10.1186/1471-2229-10-82
Rao AN, 1997. Tissue culture in the orchid industry. In: Reinert J, Bajaj YPS (Eds.), Applied and Fundamental Aspects of Plant Cell Tissue and Organ Culture. Springer, New York, USA, p.44–49.
Sheelavantmath SS, Murthy HN, Pyati AN, et al., 2000. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue Organ Cult, 60(2):151–154. https://doi.org/10.1023/A:1006426905052
Shimasaki K, 1993. Induction of Protocorm-like Body (PLB) and subsequent plantlet formation from floret culture of Neofinetia falcata Hu. Plant Tissue Cult Lett, 10(1): 75–77. https://doi.org/10.5511/plantbiotechnology1984.10.75
Stewart SL, Kane MK, 2006. Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Organ Cult, 86(2):147–158. https://doi.org/10.1007/s11240-006-9098-y
Teixeira da Silva JA, Cardoso JC, Dobránszki J, et al., 2015. Dendrobium micropropagation: a review. Plant Cell Rep, 34(5):671–704. https://doi.org/10.1007/s00299-015-1754-4
Thornhill A, Koopowitz H, 1992. Viability of Disa uniflora Berg (Orchidaceae) seeds under variable storage conditions: is orchid gene-banking possible? Biol Conserv, 62(1):21–27. https://doi.org/10.1016/0006-3207(92)91148-L
Withner CL, 1959. The Orchid: A Scientific Survey. Ronald Press Company, New York, USA, p.648.
Wotavová-Novotná K, Vejsadová H, Kindlmann P, 2007. Effects of sugars and growth regulators on in vitro growth 1004 of Dactylorhiza species. Biol Plant, 51(1):198–200. https://doi.org/10.1007/s10535-007-0040-x
Yasugi S, Shinto H, 1994. Formation of multiple shoots and regenerated plantlets by culture of pseudobulb segment in nobile type Dendrobium. Plant Tissue Cult Lett, 11(2): 153–156. https://doi.org/10.5511/plantbiotechnology1984.11.153
Zettler LW, McInnis TM, 1993. Symbiotic seed germination and development of Spiranthes cernua and Goodyera pubescens (Orchidaceae: Spiranthoideae). Lindleyana, 8(3): 155–162.
Author information
Authors and Affiliations
Contributions
Xiao-fen LIU and Li-li XIANG summarized the experimental reports and wrote the manuscript. Yan HUANG carried out the tissue culture experiment. Xiao-fen LIU, Ya-jing LI, and Fang LI analyzed the data. Fang LI has received research grants and designed this experiment. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
Corresponding author
Additional information
Compliance with ethics guidelines
Xiao-fen LIU, Li-li XIANG, Yan HUANG, Ya-jing LI, and Fang LI declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
Project supported by the Major Science and Technology Special Agricultural Projects in Zhejiang Province (No. 2007C12064) and the Zhejiang Province Breeding New Flower Varieties Major Science and Technology Key Projects (No. 2012C12909-10), China (id)
