Abstract
Seed vigor is a key factor affecting seed quality. The mechanical drying process exerts a significant influence on rice seed vigor. The initial moisture content (IMC) and drying temperature are considered the main factors affecting rice seed vigor through mechanical drying. This study aimed to determine the optimum drying temperature for rice seeds according to the IMC, and elucidate the mechanisms mediating the effects of drying temperature and IMC on seed vigor. Rice seeds with three different IMCs (20%, 25%, and 30%) were dried to the target moisture content (14%) at four different drying temperatures. The results showed that the drying temperature and IMC had significant effects on the drying performance and vigor of the rice seeds. The upper limits of drying temperature for rice seeds with 20%, 25%, and 30% IMCs were 45, 42, and 38 °C, respectively. The drying rate and seed temperature increased significantly with increasing drying temperature. The drying temperature, drying rate, and seed temperature showed extremely significant negative correlations with germination energy (GE), germination rate, germination index (GI), and vigor index (VI). A high IMC and drying temperature probably induced a massive accumulation of hydrogen peroxide (H2O2) and superoxide anions in the seeds, enhanced superoxide dismutase (SOD) and catalase (CAT) activity, and increased the abscisic acid (ABA) content. In the early stage of seed germination, the IMC and drying temperature regulated seed germination through the metabolism of H2O2, gibberellin acid (GA), ABA, and α-amylase. These results indicate that the metabolism of reactive oxygen species (ROS), antioxidant enzymes, GA, ABA, and α-amylase might be involved in the mediation of the effects of drying temperature on seed vigor. The results of this study provide a theoretical basis and technical guidance for the mechanical drying of rice seeds.
摘要
目的
本研究旨在根据种子初始含水量确定水稻种子的 最佳干燥温度, 并阐明干燥温度和种子初始水分 影响水稻种子干燥后种子活力的机制.
创新点
根据不同初始水分的水稻种子, 设置了不同梯度 的干燥温度, 系统地研究了干燥温度和种子初始 水分对种子机械干燥过程与种子活力的影响, 并 得到了不同初始水分的适宜干燥温度.
方法
在不同天气, 分别收获初始含水量为20%、25% 与30%的水稻种子样品, 并分别设置4 个温度梯 度的干燥温度。采用三九远红外线干燥机 (NP-120e)干燥水稻种子,对干燥后的水稻种 子进行标准发芽试验, 并测定相关生理生化指标, 并进行相关性分析.
结论
初始含水量为20%、25%与30%的水稻种子的适 宜干燥温度分别为45、42 与38 °C。较高的干燥 温度显著降低了水稻种子的活力。种子干燥过程 与萌发初期水稻种子内部活性氧, 抗氧化酶、赤 霉素、脱落酸和α-淀粉酶的代谢可能与干燥温度 调控水稻种子活力的机制密切相关.
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Yu-tao HUANG performed the experiments, analyzed the data, and drafted the manuscript. Wei WU and Wen-xiong ZOU contributed to the seed germination test. Hua-ping WU performed the experiments of HPLC analysis. Dong-dong CAO conceived and designed the whole experiment and revised the manuscript. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Yu-tao HUANG, Wei WU, Wen-xiong ZOU, Hua-ping WU, and Dong-dong CAO declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
Project supported by the Key Research and Development Program of Zhejiang Province (No. 2019C02011) and the “San Nong Liu Fang” Science and Technology Cooperation Project of Zhejiang Province (No. 2018SNLF004), China
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Huang, Yt., Wu, W., Zou, Wx. et al. Drying temperature affects rice seed vigor via gibberellin, abscisic acid, and antioxidant enzyme metabolism. J. Zhejiang Univ. Sci. B 21, 796–810 (2020). https://doi.org/10.1631/jzus.B2000297
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DOI: https://doi.org/10.1631/jzus.B2000297