概要
在三孢布拉霉发酵番茄红素过程中, 阻断剂是必不可少的组分. 尼古丁作为目前已知的最有效的番茄红素阻断剂之一, 其在工业生产上具有巨大的实用价值. 但阻断剂作为菌体的外源毒性生物碱, 其对菌体的代谢势必有所改变. 本文以尼古丁作为阻断剂, 对其在三孢布拉霉发酵过程中的代谢影响做了初步研究. 基于气相色谱-质谱 (GC-MS) 检测技术及小分子代谢组分的主成分分析 (PCA), 证明尼古丁在三孢布拉霉菌体内严重抑制氨基酸及糖代谢, 同时脂肪酸和有机酸代谢也有所减弱, 但相对强于糖代谢. 因此, 在尼古丁作为阻断剂时, 为积累色素, 碳源的补充应以脂肪酸或甘油为主.
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We thank Liwen Bianji, Edanz Editing China (https://www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.
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Yang LIU and You-ran SHAO analyzed the data and wrote a draft of the manuscript. Zhi-ming WANG and Li-rong YANG conducted the fermentation experiments. Xiang-yu LI and Yu-zhou ZHANG completed the sample treatments and revised the description of the GC-MS analysis. Mian-bin WU and Jian-ming YAO designed the study and modified the manuscript. All authors have read and approved the final manuscript. Therefore, all authors have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Yang LIU, You-ran SHAO, Xiang-yu LI, Zhi-ming WANG, Li-rong YANG, Yu-zhou ZHANG, Mian-bin WU, and Jian-ming YAO declare that they have no conflict of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
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Project supported by the National Key Research and Development Program of China (No. 2018YFC1604102)
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Liu, Y., Shao, Yr., Li, Xy. et al. Analysis of nicotine-induced metabolic changes in Blakeslea trispora by GC-MS. J. Zhejiang Univ. Sci. B 21, 172–177 (2020). https://doi.org/10.1631/jzus.B1900459
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DOI: https://doi.org/10.1631/jzus.B1900459