Abstract
Recent studies have shown that diet can affect the body’s immunity. Roughage of dairy cows consists of a variety of plant materials which make different contributions to health. This study investigated the effect of different roughages on the immunity of dairy cows. Serum, peripheral blood mononuclear cells (PBMCs), and milk samples were collected from 20 multiparous mid-lactation cows fed mixed forage (MF)- or corn straw (CS)-based diets. Expression profile analysis was used to detect the differentially expressed genes (DEGs) from PBMCs. The results showed that milk protein in the MF group increased to 3.22 g/100 ml, while that of the CS group milk was 2.96 g/100 ml; by RNA sequencing, it was found that 1615 genes were differentially expressed between the CS group and the MF group among the 24 027 analyzed probes. Gene ontology (GO) and pathway analysis of DEGs suggested that these genes (especially genes coding cytokines, chemokine and its receptors) are involved in the immune response. Results were confirmed at the protein level via detecting the levels of interleukin-2 (IL-2), IL-6, IL-10, IL-12, leptin (LEP), interferon-γ (IFN-γ), transforming growth factor-β1 (TGF-β1), and tumor necrosis factor-α (TNF-α) in peripheral blood by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay analysis. Our data supported the conclusions that the protein content in milk of the MF group was higher than that of the CS group, the CS-based diets induced more release of cytokines than the MF-based diets in dairy cows’ PBMCs, and milk protein content may be affected by cytokines.
中文概要
目的
研究不同类型的粗饲料对奶牛外周血细胞因子的影响。
创新点
明确饲喂玉米秸秆日粮奶牛外周血单核细胞基因的表达特征,研究日粮对外周血细胞因子的影响。
方法
分别收集饲喂苜蓿混合粗饲料和玉米秸秆粗饲料的奶牛牛奶、外周血单核细胞及血清,检测牛奶营养含量,应用表达谱基因芯片分析筛选外周血单核细胞中差异表达基因,并采用荧光定量聚合 酶链反应(qPCR)验证。最后采用酶联免疫吸附 试验(ELISA)检测血清细胞因子的表达量。
结论
与苜蓿混合粗饲料组相比较,玉米秸秆粗饲料组的奶牛外周血单核细胞炎性细胞因子分泌水平较高,牛奶乳蛋白含量较低(P<0.05)。
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We thank former laboratory members (College of Veterinary Medicine, Jilin University, China) for their contributions to the materials and technical assistance.
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Project supported by the National Natural Science Foundation of China (No. 31772715) and the National Basic Research Program (973) of China (No. 2011CB100805)
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Che, Yy., Xia, Xj., He, Bp. et al. A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows. J. Zhejiang Univ. Sci. B 19, 796–806 (2018). https://doi.org/10.1631/jzus.B1700571
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DOI: https://doi.org/10.1631/jzus.B1700571