中文概要
目的
分析H9N2 禽流感病毒通过基因重配形成H7N9 和H10N8 人间禽流感病毒的进程,探讨作为供体 的禽流感病毒H9N2 在当前中国的主要分布及其 内部6 个基因节段的进化关系。
创新点
人间禽流感病毒H7N9 和H10N8 共起源于H9N2 禽流感病毒早已成为共识,但共起源的时间节 点、作为供体的禽流感病毒H9N2 在当前中国的 分布及其内部6 个基因节段的进化关系鲜有论 及。2014 年,H5N6 禽流感被多次报道造成人类 感染。研究表明,H5N6 禽流感具有复杂的重配 来源,H9N2 正是其一,加之H7N9 第五波流行 的严峻形势,亟需明确H9N2 禽流感病毒通过基 因重配形成新亚型的能力以及它在我国的当前 主要分布。
方法
从流感病毒公共数据库下载基因序列,评估查找 适当的碱基替代模型,通过进化树查看与H7N9、 H10N8 及H5N6 具有高度相似性的H9N2 病毒的 分布地区以及它们在内部6 个基因节段上的进化 关系,同时通过碱基替代速率的计算追溯最近共 同祖先(tMRCA)及其分歧时间。
结论
人间禽流感病毒H7N9 与H10N8 均在2012 年之 前形成,短期内通过碱基替代与基因重配形成了 两种可感染人类的禽流感病毒,证实了H9N2 通 过重配形成新亚型的高效性。作为重配供体的 H9N2 至今仍广布于华东、华南及东南亚。其内 部基因节段的重配复杂,发生在亚型内部的重配 以及通过重配形成新的病毒亚型的风险都很高, 需在禽畜中加强流感病毒的流行动态监测,特别 是那些一向被忽视的编码内部蛋白的基因组节 段。
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The two authors contributed equally to this work.
Project supported by the National Key Research and Development Program of China (No. 2017YFC1200203), the Open Fund of Shanghai Key Laboratory of Meteorology and Health (No. QXJK201605), the National Natural Science Foundation of China (No. 71363001), the Fourth Round of Three-Year Public Health Action Plan of Shanghai (Nos. 15GWZK0101 and 15GWZK0202), China
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Chang, Hp., Peng, L., Chen, L. et al. Avian influenza viruses (AIVs) H9N2 are in the course of reassorting into novel AIVs. J. Zhejiang Univ. Sci. B 19, 409–414 (2018). https://doi.org/10.1631/jzus.B1700374
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DOI: https://doi.org/10.1631/jzus.B1700374