Abstract
The aim of this study is to assess the antibacterial and anti-biofilm properties of the lipid extract from Mantidis ootheca against the gentamycin resistant Pseudomonas aeruginosa. The chemical composition of the lipid extract and its relative proportion were determined using the technique of gas chromatography coupled with mass spectrometry (GC-MS). Antibacterial susceptibility tests were performed using a disc diffusion assay and the minimum inhibition concentration (MIC) was determined by way of the agar dilution method. The anti-biofilm test was carried out with crystal violet staining and scanning electron microscopy (SEM). There were 16 compounds detected, and the most abundant components were sesquiterpenoids, monoterpenes, and trace aromatic compounds. The MIC for P. aeruginosa was 4 mg/ml and the eradication effect on preformed biofilms was established and compared with a ciprofloxacin control. The results of our study indicated that a lipid extract from M. ootheca could be used as a topical and antibacterial agent with anti-biofilm activity in the future.
中文概要
目的
探究桑螵蛸脂类提取物的成分及对铜绿假单胞菌 的抗菌和抗生物膜作用。
创新点
中国传统中药桑螵蛸一直广泛应用于肾病的治 疗,在抗菌领域未见报道,本实验首次证明桑螵 蛸脂类提取物对铜绿假单胞菌有明显的抗菌和 抗生物膜作用。
方法
采用气相色谱-质谱联用技术,测定桑螵蛸脂质 提取物的化学成分及其相对比例。采用纸片扩散 法和琼脂平板稀释法观察桑螵蛸脂类提取物对 铜绿假单胞菌的抑菌效应并测定最小抑制浓度 (MIC)。采用结晶紫染色法和扫描电镜(SEM) 进行抑制生物被膜的试验。
结论
桑螵蛸脂类提取物中含有16 种化合物,最丰富 的成分分别是倍半萜类化合物、单萜和微量芳香 族化合物。桑螵蛸脂类提取物对铜绿假单胞菌的 MIC 为4 mg/ml,对铜绿假单胞菌生物被膜的抑 制作用明显。
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Artini M, Papa R, Barbato G, et al., 2012. Bacterial biofilm formation inhibitory activity revealed for plant derived natural compounds. Bioorg Med Chem, 20(2):920–926. https://doi.org/10.1016/j.bmc.2011.11.052
Baker DD, Chu M, Oza U, et al., 2007. The value of natural products to future pharmaceutical discovery. Nat Prod Rep, 24(6):1225–1244. https://doi.org/10.1039/b602241n
Berendonk TU, Manaia CM, Merlin C, et al., 2015. Tackling antibiotic resistance: the environmental framework. Nat Rev Microbiol, 13(5):310–317. https://doi.org/10.1038/nrmicro3439
Chung PY, Toh YS, 2014. Anti-biofilm agents: recent breakthrough against multi-drug resistant Staphylococcus aureus. Pathog Dis, 70(3):231–239. https://doi.org/10.1111/2049-632X.12141
Clementi EA, Wilhelm KR, Schleucher J, et al., 2013. A complex of equine lysozyme and oleic acid with bactericidal activity against Streptococcus pneumoniae. PLoS ONE, 8(11):e80649. https://doi.org/10.1371/journal.pone.0080649
Costerton JW, Lewandowski Z, Caldwell DE, et al., 1995. Microbial biofilms. Ann Rev Microbiol, 49:711–745. https://doi.org/10.1146/annurev.mi.49.100195.003431
Costerton JW, Stewart PS, Greenberg EP, 1999. Bacterial biofilms: a common cause of persistent infections. Science, 284(5418):1318–1322.
Davies D, 2003. Understanding biofilm resistance to antibacterial agents. Nat Rev Drug Discov, 2(2):114–122. https://doi.org/10.1038/nrd1008
de Zoysa GH, Cameron AJ, Hegde VV, et al., 2015. Antimicrobial peptides with potential for biofilm eradication: synthesis and structure activity relationship studies of battacin peptides. J Med Chem, 58(2):625–639. https://doi.org/10.1021/jm501084q
Gellatly SL, Hancock RE, 2013. Pseudomonas aeruginosa: new insights into pathogenesis and host defenses. Pathog Dis, 67(3):159–173. https://doi.org/10.1111/2049-632X.12033
Joo HS, Otto M, 2012. Molecular basis of in vivo biofilm formation by bacterial pathogens. Chem Biol, 19(12): 1503–1513. https://doi.org/10.1016/j.chembiol.2012.10.022
Kim J, Park HD, Chung S, 2012. Microfluidic approaches to bacterial biofilm formation. Molecules, 17(8):9818–9834. https://doi.org/10.3390/molecules17089818
Kwiecinski J, Eick S, Wojcik K, 2009. Effects of tea tree (Melaleuca alternifolia) oil on Staphylococcus aureus in biofilms and stationary growth phase. Int J Antimicrob Agents, 33(4):343–347. https://doi.org/10.1016/j.ijantimicag.2008.08.028
Lam J, Chan R, Lam K, et al., 1980. Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect Immun, 28(2):546–556.
Li J, Wu HQ, Liu ZG, 2009. Acaricidal activity of clove bud oil against Dermatophagoides farinae (Acari: Pyroglyphidae). Chin J Parasitol Parasit Dis, 27(6):492–493, 497 (in Chinese).
Luis A, Breitenfeld L, Ferreira S, et al., 2014. Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts. Pharmacogn Mag, 10(Suppl 1): S6-S13. https://doi.org/10.4103/0973-1296.127331
Murphy K, Park AJ, Hao Y, et al., 2014. Influence of O polysaccharides on biofilm development and outer membrane vesicle biogenesis in Pseudomonas aeruginosa PAO1. J Bacteriol, 196(7):1306–1317. https://doi.org/10.1128/JB.01463-13
Musa AM, Ibrahim MA, Aliyu AB, et al., 2015. Chemical composition and antimicrobial activity of hexane leaf extract of Anisopus mannii (Asclepiadaceae). J Intercult Ethnopharmacol, 4(2):129–133. https://doi.org/10.5455/jice.20150106124652
O'Donnell F, Smyth TJP, Ramachandran VN, et al., 2010. A study of the antimicrobial activity of selected synthetic and naturally occurring quinolones. Int J Antimicrob Agents, 35(1):30–38. https://doi.org/10.1016/j.ijantimicag.2009.06.031
Oliver A, Mulet X, Lopez-Causape C, et al., 2015. The increasing threat of Pseudomonas aeruginosa high-risk clones. Drug Resist Updat, 21–22:41–59. https://doi.org/10.1016/j.drup.2015.08.002
Orhan DD, Ozcelik B, Ozgen S, et al., 2010. Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiol Res, 165(6):496–504. https://doi.org/10.1016/j.micres.2009.09.002
Papa R, Selan L, Parrilli E, et al., 2015. Anti-biofilm activities from marine cold adapted bacteria against staphylococci and Pseudomonas aeruginosa. Front Microbiol, 6:1333. https://doi.org/10.3389/fmicb.2015.01333
Patra JK, Das G, Baek KH, 2015. Chemical composition and antioxidant and antibacterial activities of an essential oil extracted from an edible seaweed, Laminaria japonica L. Molecules, 20(7):12093–12113. https://doi.org/10.3390/molecules200712093
Silby MW, Winstanley C, Godfrey SA, et al., 2011. Pseudomonas genomes: diverse and adaptable. FEMS Microbiol Rev, 35(4):652–680. https://doi.org/10.1111/j.1574-6976.2011.00269.x
Sitaram C, Rupakula RB, Reddy BN, et al., 2011. Determination of alkyl methanesulfonates in doxazosin mesylate by gas chromatography-mass spectrometer. Indian J Pharm Sci, 73(1):107–110. https://doi.org/10.4103/0250-474X.89769
Stewart PS, Costerton JW, 2001. Antibiotic resistance of bacteria in biofilms. Lancet, 358(9276):135–138. https://doi.org/10.1016/S0140-6736(01)05321-1
Sutherland IW, 2001. The biofilm matrix—an immobilized but dynamic microbial environment. Trends Microbiol, 9(5): 222–227. https://doi.org/10.1016/S0966-842X(01)02012-1
Tan Z, Lei Y, Zhang B, et al., 1997. Comparison of pharmacological studies on Ootheca Mantidis. China J Chin Mater Med, 22(8):496–499 (in Chinese).
Tu YY, 2011. The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine. Nat Med, 17(10):1217–1220. https://doi.org/10.1038/nm.2471
Wang W, Chanda W, Zhong M, 2015. The relationship between biofilm and outer membrane vesicles: a novel therapy overview. FEMS Microbiol Lett, 362(15): fnv117. https://doi.org/10.1093/femsle/fnv117
Wen LL, Wan DG, Ren Y, et al., 2013. Corresponding relationship between Mantis and Mantidis oötheca (Sangpiaoxiao). China J Chin Mater Med, 38(7):966–968 (in Chinese).
Wolcott RD, Rhoads DD, Bennett ME, et al., 2010. Chronic wounds and the medical biofilm paradigm. J Wound Care, 19(2):45–46. https://doi.org/10.12968/jowc.2010.19.2.46966
Yang L, Liu Y, Wu H, et al., 2012. Combating biofilms. FEMS Immunol Med Microbiol, 65(2):146–157. https://doi.org/10.1111/j.1574-695X.2011.00858.x
Acknowledgements
We sincerely thank Prof. Jing WANG (Dalian University, Dalian, China) for providing technical assistance for this study.
Author information
Authors and Affiliations
Corresponding authors
Additional information
The two authors contributed equally to this work
Rights and permissions
About this article
Cite this article
Wang, Wd., Zhang, Nn., Chanda, W. et al. Antibacterial and anti-biofilm activity of the lipid extract from Mantidis ootheca on Pseudomonas aeruginosa. J. Zhejiang Univ. Sci. B 19, 364–371 (2018). https://doi.org/10.1631/jzus.B1700356
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1631/jzus.B1700356