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Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection

高脂饮食联合低剂量链脲佐菌素造大鼠二型糖尿病模型稳定性的研究

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Abstract

Objective

The present study aims at determining the stability of a popular type 2 diabetes rat model induced by a high-fat diet combined with a low-dose streptozotocin injection.

Methods

Wistar rats were fed with a high-fat diet for 8 weeks followed by a one-time injection of 25 or 35 mg/kg streptozotocin to induce type 2 diabetes. Then the diabetic rats were fed with regular diet/high-fat diet for 4 weeks. Changes in biochemical parameters were monitored during the 4 weeks.

Results

All the rats developed more severe dyslipidemia and hepatic dysfunction after streptozotocin injection. The features of 35 mg/kg streptozotocin rats more resembled type 1 diabetes with decreased body weight and blood insulin. Rats with 25 mg/kg streptozotocin followed by normal diet feeding showed normalized blood glucose level and pancreatic structure, indicating that normal diet might help recovery from certain symptoms of type 2 diabetes. In comparison, diabetic rats fed with high-fat diet presented decreased but relatively stable blood glucose level, and this was significantly higher than that of the control group (P>0.05).

Conclusions

This model easily recovers with normal diet feeding. A high-fat diet is suggested as the background diet in future pharmacological studies using this model.

中文概要

目的

探讨高脂饮食联合低剂量链脲佐菌素(STZ)造 大鼠二型糖尿病模型在不同饮食背景下的稳定 性。

创新点

首次探讨在正常饮食和高脂饮食的背景下,该常 见的大鼠二型糖尿病模型的稳定性。可为造模后 期干预阶段的饲料选择提供依据。

方法

饲喂Wistar 大鼠高脂饮食8 周后,注射25 或 35 mg/kg STZ 来诱导二型糖尿病。之后每组分别 饲喂正常饲料和高脂饲料4 周,检测血液生化指 标的稳定性。

结论

不管在何种饮食下,糖尿病大鼠都出现了空腹血 糖显著恢复,血脂紊乱加剧的现象。其中35 mg/kg STZ 注射组大鼠的特征更接近一型糖尿病,而 25 mg/kg STZ 注射组大鼠表现出二型糖尿病的特 征。相比高脂饮食,正常饮食更容易导致空腹血 糖和胰岛结构的恢复。因此,在正常饮食下该模 型稳定性欠佳,在后期的药理学实验中推荐使用 高脂饮食。

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Authors and Affiliations

  1. Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    Xiao-xuan Guo, Bao-ping Ji & Feng Zhou

  2. Academy of State Administration of Grain, Beijing, 100037, China

    Yong Wang

  3. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, China

    Kai Wang

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  1. Xiao-xuan Guo
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  2. Yong Wang
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  3. Kai Wang
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  4. Bao-ping Ji
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  5. Feng Zhou
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Correspondence to Feng Zhou.

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Guo, Xx., Wang, Y., Wang, K. et al. Stability of a type 2 diabetes rat model induced by high-fat diet feeding with low-dose streptozotocin injection. J. Zhejiang Univ. Sci. B 19, 559–569 (2018). https://doi.org/10.1631/jzus.B1700254

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