Abstract
Phenolics, as the main bioactive compounds in tea, have been suggested to have potential in the prevention of various human diseases. However, little is known about phenolics and their bioactivity in Zhangping Narcissue tea cake which is considered the most special kind of oolong tea. To unveil its bioactivity, three phenolic-enriched extracts were obtained from Zhangping Narcissue tea cake using ethyl acetate, n-butanol, and water. Their main chemical compositions and in vitro bioactivity were analyzed by high-performance liquid chromatography (HPLC) and ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). The ethyl acetate fraction (ZEF) consisted of higher content of phenolics, flavonoids, procyanidins, and catechin monomers (including epigallocatechin gallate (EGCG), epicatechin gallate (ECG), and gallocatechin gallate (GCG)) than n-butanol fraction (ZBF) and water fraction (ZWF). ZEF exhibited the strongest antioxidant capacity in vitro due to its abundant bioactive compounds. This was validated by Pearson correlation and hierarchical clustering analyses. ZEF also showed a remarkable inhibition on the growth, migration, and invasion of 4T1 murine breast cancer cells.
概要
目的
探讨不同提取方法所得的漳平水仙饼茶中提取物 的主要化学成分、体外抗氧化作用和对小鼠乳腺 癌4T1 细胞增殖和转移的抑制作用。
创新点
首次对漳平水仙饼茶中不同提取物的主要化学成 分、体外抗氧化作用和对小鼠乳腺癌4T1 细胞增 殖和转移的抑制作用进行了研究。
方法
以漳平水仙饼茶为原料,利用乙酸乙酯、正丁醇 和水分别进行萃取,得到三种提取物。对提取物 的茶多酚、黄酮、原花青素、总糖、蛋白质、氨 基酸、儿茶素单体、茶黄素、咖啡碱等主要化学 成分进行检测。利用DPPH、ABTS 和FRAP 三 种不同方法进一步检测了三种提取物的体外抗 氧化性,并评价了提取物对小鼠乳腺癌4T1 细胞 的增殖、细胞周期、迁移、侵袭等的影响作用。 同时,采用皮尔森系数分析和聚类分析两种分析 方法探讨了提取物中的化学成分与其抗氧化活 性和对4T1 细胞作用的相关性。
结论
本实验结果显示,漳平水仙饼茶乙酸乙酯层提取 物具有最高的茶多酚、黄酮、原花青素和儿茶素 单体的含量(表1~3,图1),并且在三种不同 的抗氧化体系中均显示出最强的抗氧化性能 (表4,图2),同时对小鼠乳腺癌4T1 细胞的 增殖、细胞周期、迁移、侵袭有非常明显的抑制 作用(图3~6)。乙酸乙酯层提取物之所以具有 最强的生物活性主要与其含有较多的茶多酚等 活性成分有关(表5,图3)。
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Project supported by the Science and Technology Department of Guangdong Province, China (No. 2016B090918118)
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In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells
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Ying, L., Kong, Dd., Gao, Yy. et al. In vitro antioxidant activity of phenolic-enriched extracts from Zhangping Narcissus tea cake and their inhibition on growth and metastatic capacity of 4T1 murine breast cancer cells. J. Zhejiang Univ. Sci. B 19, 199–210 (2018). https://doi.org/10.1631/jzus.B1700162
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DOI: https://doi.org/10.1631/jzus.B1700162