Abstract
With the increasing occurrence of haze during the summer, the physicochemical characteristics and toxicity differences in PM2.5 in different seasons are of great concern. Hangzhou is located in an area that has a subtropical monsoon climate where the humidity is very high during both the summer and winter. However, there are limited studies on the seasonal differences in PM2.5 in these weather conditions. In this test, PM2.5 samples were collected in the winter and summer, the morphology and chemical composition of PM2.5 were analyzed, the toxicity of PM2.5 to human bronchial cells BEAS-2B was compared, and the correlation between PM2.5 toxicity and the chemical composition was discussed. The results showed that during both the winter and summer, the main compounds in the PM2.5 samples were water-soluble ions, particularly SO42−, NO3−, and NH4+, followed by organic components, while heavy metals were present at lower levels. The higher the mass concentration of PM2.5, the greater its impact on cell viability and ROS levels. However, when the mass concentration of PM2.5 was similar, the water extraction from the summer samples showed a greater impact on BEAS-2B than that from the winter samples. The cytotoxicity of PM2.5 was closely associated with heavy metals and organic pollutants but less related to water-soluble ions.
概 要
创新点
对东南沿海地区典型亚热带季风气候影响下的不同季节 PM2.5 颗粒物的水提液进行了毒性探究。
方 法
对比了冬夏 PM2.5 样品的化学成分, 及其水提液对人肺支气管细胞 BEAS-2B 的毒性, 并探讨了其毒性和化学组成间的相关性。
结 论
冬夏季节 PM2.5 样品均以水溶性离子, 尤其是 SO42−、 NO3− 和 NH4+为主, 其次是有机组分, 重金属含量较少。 PM2.5 浓度越高, 暴露时对细胞活力的影响越大, 对细胞内活性氧 (ROS) 增加的影响也越大。 但是当 PM2.5 浓度相当时, 夏季采集的 PM2.5的暴露液对细胞活力和细胞内 ROS 水平的影响更大。 PM2.5 的细胞毒性与重金属和有机污染物关系密切, 而与质量比重最高的水溶性离子关系不大。
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Project supported by the National Natural Science Foundation of China (No. 41371447) and the National Key Technologies R&D Program of China (No. 2013BAC16B04)
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Zhang, Hh., Li, Z., Liu, Y. et al. Physical and chemical characteristics of PM2.5 and its toxicity to human bronchial cells BEAS-2B in the winter and summer. J. Zhejiang Univ. Sci. B 19, 317–326 (2018). https://doi.org/10.1631/jzus.B1700123
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DOI: https://doi.org/10.1631/jzus.B1700123