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Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry

HPLC-LTQ-Orbitrap 方法分析丹参的代谢轮廓

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Journal of Zhejiang University-SCIENCE B Aims and scope Submit manuscript

Abstract

Danshen, the dried root of Salvia miltiorrhiza Bunge (Lamiaceae), is one of the traditional Chinese medicines (TCMs) most commonly used for the treatment of cardiovascular and cerebrovascular diseases. However, little is known about the chemical and metabolic profiles of danshen in vitro or in vivo. In particular, more information is needed in relation to the 50% ethanol extracts usually used in danshen formulations such as Fufang Xueshuantong Capsules and Fufang Danshen tablets. High-performance liquid chromatography coupled with a linear ion trap- Orbitrap mass spectrometer (HPLC-LTQ-Orbitrap) provides a sensitive and accurate method for analyzing the composition of samples. This method was used to determine the in vitro and in vivo chemical and metabolic profiles of danshen. Sixty-nine components of danshen extract and 118 components of danshen in rat plasma, urine, feces, and bile were unambiguously or tentatively identified. These results not only revealed the material composition of danshen, but also provided a comprehensive research approach for the identification of multi-constituents in TCMs.

概要

目的

对丹参50%乙醇提取物的体内外物质基础进行系 统全面的分析。

创新点

首次采用高效液相色谱-质谱联用(HPLC-LTQOrbitrap) 方法对丹参50%乙醇提取物的体内外 化学和代谢成分进行全面分析。

方法

建立HPLC-LTQ-Orbitrap 方法,对丹参50%乙醇提取物的化学成分以及给药后大鼠血浆、尿液、粪便和胆汁生物样品中的化学和代谢成分进行分析。

结论

在正负离子模式下,在丹参提取物中鉴定出共69个化合物,包括丹酚酸类化合物23 个,丹参酮类化合物33 个,以及未知化合物13 个;在大鼠灌胃给予丹参提取物后的生物样本中鉴定出共118 个化合物,包括血浆中35 个,尿液中63 个,粪便中62 个,以及胆汁中18 个。

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Authors and Affiliations

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100102, China

    Huan-huan Pang, Mei-fang Jiang, Qin-hui Wang, Xiao-ye Wang, Wei Gao, Zhi-hao Tian & Jian-mei Huang

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  1. Huan-huan Pang
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  2. Mei-fang Jiang
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  3. Qin-hui Wang
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  4. Xiao-ye Wang
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  5. Wei Gao
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  6. Zhi-hao Tian
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  7. Jian-mei Huang
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Correspondence to Jian-mei Huang.

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Project supported by the Ministry of Science and Technology of China (No. 2011ZX09201-201-22)

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Pang, Hh., Jiang, Mf., Wang, Qh. et al. Metabolic profile of danshen in rats by HPLC-LTQ-Orbitrap mass spectrometry. J. Zhejiang Univ. Sci. B 19, 227–244 (2018). https://doi.org/10.1631/jzus.B1700105

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