Creation of a rabbit model for intrauterine adhesions using electrothermal injury

Abstract

The pathogenesis and therapeutic treatment of intrauterine adhesions (IUAs) remain unsolved, highlighting the need for stable and effective experimental animal models. In this study, uterine electrocoagulation of twenty-one female New Zealand White rabbits was carried out to establish an IUA model. As rabbits have two completely separate uterine horns, each rabbit had its own internal control: one uterine horn was given an electrothermal injury (Group A, n=21), and the contralateral uterine horn received no treatment and served as the control (Group B, n=21). The endometrial morphology, number of endometrial glands, area of endometrial fibrosis, and number of implanted fetuses were compared between the two groups. In Group A, the numbers of endometrial glands on Days 7 and 14 and the number of implanted fetuses were significantly lower than those in Group B (P<0.05, P<0.05, and P<0.01, respectively), while the ratio of the area with endometrial stromal fibrosis to the total endometrial area was significantly increased (P<0.01). These results suggest that this method of electrothermal injury is effective for the establishment of a rabbit IUA model between 7 and 14 d after surgery.

中文概要

目的

目前宫腔粘连(Intrauterine adhesion,IUA)发病 机制及治疗方法仍未明确和统一,建立一个稳定 有效的宫腔粘连动物模型是开展相关研究的前 提和基础。本文旨在利用电热损伤法构建兔IUA 模型,观察和评估该方法的建模效果。

创新点

首次提出利用电热损伤法建立兔宫腔粘连模型, 并得出在损伤后7~14 天内建立的兔IUA 模型是 有效的结论。

方法

将21 只成年雌性新西兰大白兔一侧子宫内膜用 医用多功能高频电刀电灼损伤模拟宫腔粘连形 成(A 组,y=n=21),另一侧子宫不做处理作为自 身对照(B 组,n=21)。分别在损伤后7、14 和 28 天收集兔双侧子宫组织,行苏木精-伊红染色 法(HE)和Masson 染色观察两侧子宫内膜病理 改变,并对两侧子宫内膜的腺体个数和内膜纤维 化面积比进行统计学分析和比较。另外,将损伤 后7 天的雌兔与成年雄兔合笼, 14 天后观察比 较两侧子宫胚胎个数。

结论

病理组织学观察显示,损伤后7 和14 天,A 组子 宫内膜腺体数量较B 组减少,差异均有统计学意 义(P<0.05);而损伤后30 天,A 组子宫内膜腺 体数量与B 组相比差异无统计学意义。损伤后7 天,A 组内膜纤维化面积较B 组增大,差异有统 计学意义(P<0.01);A 组子宫胚胎个数较B 组 减少,差异有统计学意义(P<0.01)。综上所述, 采用电热损伤法建立的兔宫腔粘连模型在损伤 后7~14 天是稳定有效的。