Skip to main content

Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats

蜡菊乙醇提取物对庆大霉素诱导的大鼠肾毒性的影响

Journal of Zhejiang University-SCIENCE B volume 18, pages 501–511 (2017)Cite this article

Abstract

The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. Gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters.

摘要

目的

评估蜡菊乙醇提取物(HPE)对庆大霉素诱导的 肾毒性的治疗或保护作用。

方法

将36 只体重200~250 g 的Sprague Dawley 雄性大 鼠分成6 组,每组6 只,适应实验室条件7 d。 每组处理方式不同,包括:组I,对照组,5% DMSO;组II,HPE 100 mg/(kg·d);组III,HPE 200 mg/(kg·d);组IV,庆大霉素80 mg/(kg·d); 组V,庆大霉素80 mg/(kg·d)+HPE 100 mg/(kg·d);组VI,庆大霉素80 mg/(kg·d)+HPE 200 mg/(kg·d)。 腹腔注射8 d 后,取血清、肝和肾组织用于评估 血液尿素氮(BUN)、肌酐、酶和非酶抗氧化剂 和脂质过氧化。

结论

庆大霉素能显著提升血清BUN、肌酐和肝肾阳 性以及丙二醛(MDA)水平,同时降低过氧化 氢酶(CAT),谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性。用100 mg/kg HPE 的治疗能逆转庆大霉素诱导的改变。因此, 100 mg/kg HPE 在肾脏和肝脏中可作为自由基和 清除剂,具有缓解庆大霉素在生物化学和组织病 理学上毒性的作用。

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Ademiluyi, A.O., Oboh, G., Owoloye, T.R., et al., 2013. Modulatory effect of dietary inclusion of garlic (Allium Sativum) on gentamicin-induced hepatotoxicity and oxidative stress in rats. Asian. Pac. J. Trop. Biomed., 3(6): 470–475. http://dx.doi.org/10.1016/S2221-1691(13)60098-2

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Albayrak, S., Aksoy, A., Sagdic, O., et al., 2009. Composition, antioxidant and antimicrobial activities of Helicrysum (Asteraceae) species collected from Turkey. Food Chem., 119(1):114–122. http://dx.doi.org/10.1016/j.foodchem.2009.06.003

    Article  Google Scholar 

  • Ali, B.H., 2003. Agents ameliorating or augmenting experimental gentamicin nephrotoxicity: some recent research. Food Chem. Toxicol., 41(11):1447–1452. http://dx.doi.org/10.1016/S0278-6915(03)00186-8

    CAS  PubMed  Article  Google Scholar 

  • Al-Kenanny, E.R., Al-Hayaly, L.K., Al-Badrany, A.G., 2012. Protective effect of Arabic gum on liver injury experimentally induced by gentamicin in mice. Kufa J. Vet. Med. Sci., 3:174–189.

    Google Scholar 

  • Aslan, M., 2000. Seker hastaligina karsi halkilaci olarak kullanilan bitkiler üzerinde Farmakognozik Arastirmalar. Doktora tezi, Gazi Üniversitesi Saglik Bilimler Enstitüsü farmakognozik Anabilimdali, Ankara, p.216 (in Turkish).

    Google Scholar 

  • Aslan, M., Orhan, D.D., Orhan, N., et al., 2007. In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induceddiabetic rats. J. Ethnopharmacol., 109(1):54–59. http://dx.doi.org/10.1016/j.jep.2006.07.001

    PubMed  Article  Google Scholar 

  • Balakumar, P., Rohilla, A., Thangathirupathi, A., 2010. Gentamicin-induced nephrotoxicity: do we have a promising therapeutic approach to blunt it? Pharmacol. Res., 62(3):179–186. http://dx.doi.org/10.1016/j.phrs.2010.04.004

    CAS  PubMed  Article  Google Scholar 

  • Banday, A.A., Farooq, N., Priyamvada, S., et al., 2008. Time dependent effects of gentamicin on the enzymes of carbohydrate metabolism, brush border membrane and oxidative stress in rat kidney tissues. Life Sci., 82(9-10): 450–459. http://dx.doi.org/10.1016/j.lfs.2007.11.014

    CAS  PubMed  Article  Google Scholar 

  • Bayer, R.J., Breitwieaser, I., Ward, J., et al., 2007. Tribe Gnaphalieae (Cass.) Lecoq & Juillet (1831). In: Kadereit, J.W., Jeffrey, C. (Eds.), The Families and Genera of Vascular Plants, Asterales. Springer, Berlin, p.246–284.

    Google Scholar 

  • Corona, P.S., Espinal, L., Rodriguez-Pardo, D., et al., 2014. Antibiotic susceptibility in Gram-positive chronic joint arthroplasty infections: increased aminoglycoside resistance rate in patients with prior aminoglycosideimpregnated cement spacer use. J. Arthropl., 29(8):1617–1621. http://dx.doi.org/10.1016/j.arth.2014.03.029

    Article  Google Scholar 

  • El-Kashef, D.H., El-Kenawi, A.E., et al., 2015. Protective effect of allicin against gentamicin-induced nephrotoxicity in rats. Int. Immunopharmacol., 29(2):679–686. http://dx.doi.org/10.1016/j.intimp.2015.09.010

    CAS  PubMed  Article  Google Scholar 

  • Góth, L.A., 1991. Simple method for determination of serum catalase activity and revision of serum catalase activity and revision of reference range. Clin. Chim. Acta, 196(2-3):143–152. http://dx.doi.org/10.1016/0009-8981(91)90067-M

    PubMed  Article  Google Scholar 

  • Guner, A., 2012. Turkiye Bitkileri Listesi (The List of Turkish Plants), Namas Matbaacilik San. Tic. A.S., Istanbul, p.163–165 (in Turkish).

    Google Scholar 

  • Kamel, M.A., Hosny Abdel Fadil, I., Noha, M.A., 2015. Prevention of Hepato-renal toxicity with vitamin E, vitamin C and their combination in gentamicin treated rats. Int. J. Pharma Sci., 5(6):1289–1296.

    Google Scholar 

  • Kandemir, F.M., Özkaraca, M., Apaydin Yildirim, B., et al., 2015. Rutin attenuates gentamicin-induced renal damage by reducing the oxidative stress, inflammation, apoptosis and autophagy in rats. Ren. Fail., 23:1–8. http://dx.doi.org/10.3109/0886022X.2015

    Google Scholar 

  • Liu, P., Feng, Y., Dong, D., et al., 2016. Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicininduced acute kidney injury. Sci. Rep.-UK, 6:20287. http://dx.doi.org/10.1038/srep20287

    CAS  Article  Google Scholar 

  • Mahmood, N., Haleh, M., Mohammad, P., et al., 2014. Pathological changes of gentamicin in liver tissue and antioxidant property of Cinnamon extract on Wistar rats. Biomed. Pharmacol. J., 7(1):341–347. http://dx.doi.org/10.13005/bpj/496

    Article  Google Scholar 

  • Masakazu, K., Yoshiko, E., Masashi, E., 2014. Acquired resistance of Listeria monocytogenes in and escaped from liver parenchymal cells to gentamicin is caused by being coated with their plasma membrane. Microb. Infect., 16(3):237–243. http://dx.doi.org/10.1016/j.micinf.2013.11.005

    Article  Google Scholar 

  • Martínez-Salgado, C., López-Hernández, F.J., López-Novoa, J.M., 2007. Glomerular nephrotoxicity of amino nucleosides. Toxicol. Appl. Pharmacol., 223(1):86–98. http://dx.doi.org/10.1016/j.taap.2007.05.004

    PubMed  Article  Google Scholar 

  • Matkovics, B., Szabo, L., Varga, I.S., 1988. Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15:248–249 (in Hungarian).

    Google Scholar 

  • Mert, N., 1996. Veteriner Klinik Biyokimya. Uludag Universitesi Veteriner Fakultesi Guclendirme Vakfi Yayin 12, Bursa, p.151–153 (in Turkish).

    Google Scholar 

  • Moreira, M.A., Nascimento, M.A., Bozzo, T.A., et al., 2014. Ascorbic acid reduces gentamicin induced nephrotoxicity in rats through the control of reactive oxygen species. Clin. Nutr., 33(2):296–301. http://dx.doi.org/10.1016/j.clnu.2013.05.005

    CAS  PubMed  Article  Google Scholar 

  • Morone-Fortunato, I., Montemurro, C., Ruta, C., et al., 2010. Essential oils, genetic relationships and in vitro establishment of Helichrysum italicum (Roth) G. Don subsp. italicum from wild Mediterranean germplasm. Ind. Crops Prod., 32(3):639–649. http://dx.doi.org/10.1016/j.indcrop.2010.07.023

    CAS  Article  Google Scholar 

  • Nasri, H., 2012. Acute kidney injury and beyond. J. Ren. Inj. Prev., 1(1):1–2. http://dx.doi.org/10.12861/jrip.2012.01

    PubMed  PubMed Central  Google Scholar 

  • Nayma, S., Sadia, C.S.M., Tanveer, H.P., et al., 2012. Effects of Ashwagandha (Withania somnifera) root extract on some serum liver marker enzymes (AST, ALT) in gentamicin intoxicated rats. J. Bangladesh Soc. Physiol., 7(1):1–7. http://dx.doi.org/10.3329/jbsp.v7i1.11152

    Google Scholar 

  • Ojano-Dirain, C.P., Antonelli, P.J., le Prell, C.G., 2014. Mitochondria-targeted antioxidant MitoQ reduces gentamicin-induced ototoxicity. Otol. Neurotol., 35(3): 533–539. http://dx.doi.org/10.1097/MAO.0000000000000192

    PubMed  Article  Google Scholar 

  • Ouédraogo, M., Lamien-Sanoub, A., Ramdeb, N., et al., 2013. Protective effect of Moringa oleifera leaves against gentamicin-induced nephrotoxicity in rabbits. Exp. Toxicol. Pathol., 65(3):335–339. http://dx.doi.org/10.1016/j.etp.2011.11.006

    PubMed  Article  Google Scholar 

  • Peng, J., Gones, G.L., Watson, K., 2000. Stress protein as biomarkers of oxidative stress: effects of antioxidant supplement. Free Radic. Biol. Med., 28(11):1598–1606. http://dx.doi.org/10.1016/S0891-5849(00)00276-8

    CAS  PubMed  Article  Google Scholar 

  • Placer, Z.A., Cushmanni, L.L., Johnson, B.C., 1966. Estimation of products of lipid peroxidation (as malondialdehyde) in biochemical systems. Anal. Biochem., 16(2): 359–364. http://dx.doi.org/10.1016/0003-2697(66)90167-9

    CAS  PubMed  Article  Google Scholar 

  • Raju, S., Kavimani, S., Maheshwara rao, V.U., et al., 2011. Floral extract of Tecoma stans: a potent inhibitor of gentamicin-induced nephrotoxicity in vivo. Asian Pac. J. Trop. Med., 4(9):680–685. http://dx.doi.org/10.1016/S1995-7645(11)60173-9

    CAS  PubMed  Article  Google Scholar 

  • Sagdic, O., Karahan, A.G., Ozcan, M., et al., 2003. Effect of some spice extracts on bacterial inhibition. Food Sci. Technol. Int., 9(5):353–356. http://dx.doi.org/10.1177/1082013203038976

    Article  Google Scholar 

  • Sala, A., Recio, M.C., Schinella, G.R., et al., 2003. Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside. Eur. J. Pharmacol., 461(1):53–61. http://dx.doi.org/10.1016/S0014-2999(02)02953-9

    CAS  PubMed  Article  Google Scholar 

  • Salem, E.A., Salem, N.A., Kamel, M., et al., 2010. Amelioration of gentamicin nephrotoxicity by green tea extract in uninephrectomized rats as a model of progressive renal failure. Ren. Fail., 32(10):1210–1215. http://dx.doi.org/10.3109/0886022X.2010.517350

    CAS  PubMed  Article  Google Scholar 

  • Sezik, E., Yesilada, E., Honda, G., et al., 2001. Traditional medicine in Turkey. X. Folk medicine in Central Anatolia. J. Ethnopharmacol., 75(2-3):95–115. http://dx.doi.org/10.1016/S0378-8741(00)00399-8

    CAS  PubMed  Article  Google Scholar 

  • Shrestha, B., Haylor, J., 2014. Experimental rat models of chronic allograft nephropathy: a review. Int. J. Nephrol. Renovasc. Dis., 23(7):315–322. http://dx.doi.org/10.2147/IJNRD.S65604

    Article  Google Scholar 

  • Singh, A.P., Junemann, A., Muthuraman, A., et al., 2012. Animal models of acute renal failure. Pharmacol. Rep., 64(1):31–44. http://dx.doi.org/10.1016/S1734-1140(12)70728-4

    CAS  PubMed  Article  Google Scholar 

  • Smirnov, V., Preobrazheskaia, N., Kalashnikov, I., 1982. Antibacterial properties of Helichrysum plicatum. Mikrobiol. Z., 44:71–72.

    CAS  Google Scholar 

  • Stafford, G.I., Jäger, A.K., van Staden, J., 2005. Effect of storage on the chemical composition and biological activity of several popular South African medicinal plants. J. Ethnopharmacol., 97(1):107–115. http://dx.doi.org/10.1016/j.jep.2004.10.021

    CAS  PubMed  Article  Google Scholar 

  • Stojiljkovic, N., Stoiljkovic, M., 2006. Micromorphological and histochemical characteristics of a rat’s liver treated with gentamicin. Acta Medica Medianae, 45(3):24–28.

    Google Scholar 

  • Sumbul, H., Gokturk, R.S., Dussen, O.D., 2003. A new endemic species of Helichrysum Gaertn. (Asteraceae-Inuleae) from south Anatolia. Bot. J. Linn. Soc., 141(2):251–254. http://dx.doi.org/10.1046/j.1095-8339.2003.00109.x

    Article  Google Scholar 

  • Sun, Y., Oberley, L.W., Li, Y.A., 1988. Simple method for clinical assay of superoxide dismutase. Clin. Chem., 34(3): 497–500.

    CAS  PubMed  Google Scholar 

  • Süzgec, S., Mericli, A.H., Houghton, P.J., et al., 2005. Flavonoids of Helichrysum compactum and their antioxidant and antibacterial activity. Fitoterapia, 76(2):269–272. http://dx.doi.org/10.1016/j.fitote.2004.12.006

    PubMed  Article  Google Scholar 

  • Tavafi, M., 2013. Protection of renal tubules against gentamicin induced nephrotoxicity. J. Ren. Inj. Prev., 2(1):5–6. http://dx.doi.org/10.12861/jrip.2013.03

    PubMed  PubMed Central  Google Scholar 

  • Tavafi, M., Ahmadvand, H., 2011. Effect of rosmarinic acid on inhibition of gentamicin induced nephrotoxicity in rats. Tissue Cell, 43(6):392–397. http://dx.doi.org/10.1016/j.tice.2011.09.001

    CAS  PubMed  Article  Google Scholar 

  • Veljkovic, M., Pavlovic, D.R., Stojiljkovic, N., et al., 2016. Morphological and morphometric study of protective effect of green tea in gentamicin-induced nephrotoxicity in rats. Life Sci., 147:85–91. http://dx.doi.org/10.1016/j.lfs.2016.01.035

    CAS  PubMed  Article  Google Scholar 

  • Wang, Y., Iwatani, H., Ito, T., et al., 2004. Fetal cells in mother rats contribute to the remodeling of liver and kidney after injury. Biochem. Bioph. Res. Commun., 325(3):961–967. http://dx.doi.org/10.1016/j.bbrc.2004.10.105

    CAS  Article  Google Scholar 

  • Yaman, I., Balikci, S., 2010. Protective effect of Nigella sativa against gentamicin-induced nephrotoxicity in rats. Exp. Toxicol. Path., 62(2):183–190. http://dx.doi.org/10.1016/j.etp.2009.03.006

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Unsal Sami AKTAS (Ani-Med Pharmacy, Turkey) for the financial support and Meryem SENGUL (Department of Botanic, Faculty of Agriculture, Ataturk University, Erzurum, Turkey) for identifying Helichrysum plicatum DC. subsp. plicatum plant.

Author information

Affiliations

  1. Department of Biochemistry, Veterinary Faculty, Ataturk University, 25240, Erzurum, Turkey

    Betul Apaydin Yildirim & Esra Aktas Senocak

  2. Department of Plant Protection, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Saban Kordali

  3. Department of Pathology, Veterinary Faculty, Ataturk University, 25240, Erzurum, Turkey

    Kubra Asena Terim Kapakin & Serdar Altun

  4. Department of Animal Science, Veterinary Faculty, Ataturk University, 25240, Erzurum, Turkey

    Fatih Yildirim

Authors
  1. Betul Apaydin Yildirim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saban Kordali
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kubra Asena Terim Kapakin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fatih Yildirim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Esra Aktas Senocak
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Serdar Altun
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Betul Apaydin Yildirim.

Additional information

ORCID: Betul APAYDIN YILDIRIM, http://orcid.org/0000-0002-0361-6148

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Apaydin Yildirim, B., Kordali, S., Terim Kapakin, K.A. et al. Effect of Helichrysum plicatum DC. subsp. plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats. J. Zhejiang Univ. Sci. B 18, 501–511 (2017). https://doi.org/10.1631/jzus.B1500291

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1631/jzus.B1500291

Key words

  • Antioxidants
  • Extract
  • Gentamicin
  • Helichrysum plicatum DC. subsp. plicatum
  • Nephrotoxicity
  • Oxidative stress

CLC number

  • R965

关键词

  • 抗氧化剂
  • 提取物
  • 蜡菊
  • 庆大霉素
  • 肾毒性
  • 氧化应激