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Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults

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Abstract

Objective

To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults.

Methods

Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples.

Results

The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). Essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash.

Conclusions

SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.

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Authors and Affiliations

  1. Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China

    Jia-yan He & Xue-ping Zhou

  2. Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, School of Medicine, Zhejiang University, Hangzhou, 310006, China

    Gang-gang Qi, Wu-jing Huang, Chun-mei Peng & Hui Chen

  3. Department of Stomatology, Hangzhou Aeromedicine Certification and Training Center of the PLA Air Force, Hangzhou, 310013, China

    Xu-dong Sun & Yu Tong

Authors
  1. Jia-yan He
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  2. Gang-gang Qi
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  3. Wu-jing Huang
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  4. Xu-dong Sun
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  5. Yu Tong
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  6. Chun-mei Peng
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  7. Xue-ping Zhou
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  8. Hui Chen
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Correspondence to Hui Chen.

Additional information

Project supported by the Zhejiang Provincial Natural Science Foundation of China (No. Y2080253), the Education Department of Zhejiang Province (No. Y200909376), the Science and Technology Department of Zhejiang Province (No. 2007C24010), and 2011 China State Key Clinical Department Grants

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He, Jy., Qi, Gg., Huang, Wj. et al. Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults. J. Zhejiang Univ. Sci. B 14, 416–425 (2013). https://doi.org/10.1631/jzus.B1200350

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  • DOI: https://doi.org/10.1631/jzus.B1200350

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