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Maize seed coatings and seedling sprayings with chitosan and hydrogen peroxide: their influence on some phenological and biochemical behaviors

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Abstract

Objective

To evaluate the effect of chitosan (CH) and hydrogen peroxide (H2O2) seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology, the H2O2 presence, the catalase (CAT) activity, and the protein quantity.

Methods

Seven groups of ten seeds for each maize variety were treated with CH (2% (20 g/L) and 0.2% (2 g/L)) or H2O2 (8 mmol/L) by coating, sprinkling, or both. Germination and seedling growth were measured. One month after germination, the presence of H2O2 in seedlings in the coated seed treatments was evaluated. Protein content and CAT activity were determined under all treatments.

Results

H2O2 seed coating enhanced the germination rate and increased seedling and stem length in the quality protein maize (QPM) variety. Seedlings had a higher emergence velocity under this treatment in both varieties. CH and H2O2 sprinklings did not have an effect on seedling phenology. Exogenous application of H2O2 promoted an increase of endogenous H2O2. CH and H2O2 seedling sprinkling increased the protein content in both maize varieties, while there was no significant effect on the CAT activity of treated seeds and seedlings.

Conclusions

CH and H2O2 enhance some phenological and biochemical features of maize depending on their method of application.

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Authors and Affiliations

  1. Laboratorio de Biotecnología, Facultad de Estudios Superiores Cuautitlán, Campo 1, Universidad Nacional Autónoma de México, Estado de México, 54740, México

    Eva-Guadalupe Lizárraga-Paulín, Susana-Patricia Miranda-Castro & Alma-Virginia Lara-Sagahón

  2. Unidad de Investigación en Granos y Semillas, Facultad de Estudios Superiores Cuautitlán, Centro de Asimilación Tecnológica, Universidad Nacional Autónoma de México, Estado de México, 54740, México

    Ernesto Moreno-Martínez

  3. CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Querétaro, 76010, México

    Irineo Torres-Pacheco

Authors
  1. Eva-Guadalupe Lizárraga-Paulín
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  2. Susana-Patricia Miranda-Castro
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  3. Ernesto Moreno-Martínez
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  4. Alma-Virginia Lara-Sagahón
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  5. Irineo Torres-Pacheco
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Correspondence to Susana-Patricia Miranda-Castro.

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Lizárraga-Paulín, EG., Miranda-Castro, SP., Moreno-Martínez, E. et al. Maize seed coatings and seedling sprayings with chitosan and hydrogen peroxide: their influence on some phenological and biochemical behaviors. J. Zhejiang Univ. Sci. B 14, 87–96 (2013). https://doi.org/10.1631/jzus.B1200270

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  • DOI: https://doi.org/10.1631/jzus.B1200270

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